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SDS-Page (SDS-PAGE: 2.5ug PRAK)

PRAK, active Active Protein | Kcnab2 active protein

PRAK, active (p38 Regulated Activated Protein Kinase)

Gene Names
Kcnab2; F5; I2rf5; Kcnb3; kv-beta-2
Purity
Highly Purified
90%. Purified using Ni-NTA agarose, activation by MAP Kinase and removal of activator on GSH-agarose.
Synonyms
PRAK; active; active (p38 Regulated Activated Protein Kinase); Kcnab2 active protein
Ordering
For Research Use Only!
Purity/Purification
Highly Purified
90%. Purified using Ni-NTA agarose, activation by MAP Kinase and removal of activator on GSH-agarose.
Form/Format
Supplied as a solution in 50mM Tris- HCl, pH 7.5, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.15M NaCl, 0.27M sucrose, and 0.02% Brij-35.
Specific Activity
~30units/mg.
Unit Definition
One unit of PRAK activity is equal to 1nmol phosphate incorporated into substrate peptide in one minute.
Preparation and Storage
-20 degree C

SDS-Page

(SDS-PAGE: 2.5ug PRAK)

SDS-Page (SDS-PAGE: 2.5ug PRAK)
Related Product Information for Kcnab2 active protein
p38-regulated/activated protein kinase (PRAK) is a 471 amino acid protein with 20-30% sequence identity to the known MAP kinase-regulated protein kinases RSK1/2/3, MNK1/2, and MAPKAP-K2/3. PRAK is activated by stress-related extra-cellular stimuli. The regulatory phosphorylation site of PRAK is Thr 182 ; PRAK is phosphorylated by p38 a and p38 ß in vitro at this residue. Activated PRAK specifically phosphorylates HSP27 at its physiologically relevant sites in vitro.

Full-length amino acid sequence corresponding to human PRAK with an N-terminal His-tag, expressed in Sf9 cells.
Mr~ 54kD.
Product Categories/Family for Kcnab2 active protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
54kD
NCBI Official Full Name
potassium channel beta 2 subunit
NCBI Official Synonym Full Names
potassium voltage-gated channel, shaker-related subfamily, beta member 2
NCBI Official Symbol
Kcnab2
NCBI Official Synonym Symbols
F5; I2rf5; Kcnb3; kv-beta-2
NCBI Protein Information
voltage-gated potassium channel subunit beta-2; neuroimmune protein F5; K(+) channel subunit beta-2; potassium voltage gated channel, shaker related subfamily, beta member 2
UniProt Protein Name
Voltage-gated potassium channel subunit beta-2
UniProt Gene Name
Kcnab2
UniProt Synonym Gene Names
Ckbeta2; I2rf5; Kcnb3
UniProt Entry Name
KCAB2_MOUSE

Uniprot Description

Kv-beta2: Accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit. Alters functional properties of Kv1.4. Forms heteromultimeric complex with alpha subunits. Forms a ternary complex with SQSTM1 and PRKCZ. Belongs to the shaker potassium channel beta subunit family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, potassium

Cellular Component: microtubule; voltage-gated potassium channel complex; neuron projection; extrinsic to internal side of plasma membrane; membrane; axon; cytoplasm; postsynaptic density; integral to membrane; nerve terminal; cytosol

Molecular Function: protein binding; voltage-gated potassium channel activity; potassium channel regulator activity; aldo-keto reductase activity; voltage-gated ion channel activity

Biological Process: protein heterooligomerization; transport; neuromuscular process; ion transport; hemopoietic progenitor cell differentiation; potassium ion transport

Research Articles on Kcnab2

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Product Notes

The Kcnab2 kcnab2 (Catalog #AAA634322) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PRAK, active, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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