Testing Data
(Kinase Assay: 0.3–1.6ng of this lot of enzyme phosphorylated 250uM cdc2 substrate peptide in the assay. Assay background was subtracted from the actual counts to yield the results shown below.)
Hck, active Active Protein
Hck, active (Hematopoietic Cellular Kinase) Recombinant
Purity
PurifiedPurified using Ni2+/NTA agarose. ~70% by SDS-PAGE and Coomassie blue staining.
Synonyms
Hck; active; active (Hematopoietic Cellular Kinase) Recombinant; active active protein
Host
Recombinant, human (Sf21 cell)
Purity/Purification
Purified
Purified using Ni2+/NTA agarose. ~70% by SDS-PAGE and Coomassie blue staining.
Purified using Ni2+/NTA agarose. ~70% by SDS-PAGE and Coomassie blue staining.
Form/Format
Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Specific Activity
As reported (typically >10,000U/mg). One unit of Hck activity is defined as 1nmol phosphate incorporated into 250uM Src substrate peptide (KVEKIGEGTYGVVYK) per minute at 30 degree C with a final ATP concentration of 100uM.
Preparation and Storage
For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Testing Data
(Kinase Assay: 0.3–1.6ng of this lot of enzyme phosphorylated 250uM cdc2 substrate peptide in the assay. Assay background was subtracted from the actual counts to yield the results shown below.)
Testing Data
(Kinase Assay: 0.3–1.6ng of this lot of enzyme phosphorylated 250uM cdc2 substrate peptide in the assay. Assay background was subtracted from the actual counts to yield the results shown below.)
SDS-Page
(SDS-PAGE and Coomassie blue staining of 3ug of H1827A)
SDS-Page
(SDS-PAGE and Coomassie blue staining of 3ug of H1827A)
Related Product Information for Hck, active active protein
N-terminal His6-tagged, recombinant, human Hck, amino acids 230 - 497, expressed by baculovirus in Sf21 insect cells.
Product Categories/Family for Hck, active active protein
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Product Notes
The Hck, active (Catalog #AAA636789) is an Active Protein produced from Recombinant, human (Sf21 cell) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Hck, active, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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