SDS-Page
(SDS-PAGE and coomassie Stain: 2.5ug of P5600-07.)
PRAK, unactive Active Protein
PRAK, unactive, Recombinant, Human (p38 Regulated Activated Protein Kinase)
Purity
Highly Purified~85%. Purified using Ni-NTA agarose.
Synonyms
PRAK; unactive; Recombinant; Human (p38 Regulated Activated Protein Kinase); unactive active protein
Host
Recombinant, human
Purity/Purification
Highly Purified
~85%. Purified using Ni-NTA agarose.
~85%. Purified using Ni-NTA agarose.
Form/Format
Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 0.15M sodium chloride, 0.1mM EGTA, 0.1% 2- mercaptoethanol, 0.02% Brij-35, 50% glycerol.
Specific Activity
~3.5units/mg after activation with SAPK2a/p38 a.
Unit Definition
One unit of PRAK activity is defined as 1nmol phosphate incorporated into substrate peptide per minute.
Preparation and Storage
Stable for 6 months at -20 degree C from date of shipment.
SDS-Page
(SDS-PAGE and coomassie Stain: 2.5ug of P5600-07.)
SDS-Page
(SDS-PAGE and coomassie Stain: 2.5ug of P5600-07.)
Related Product Information for PRAK, unactive active protein
p38-regulated/activated protein kinase (PRAK) is a 471 amino acid protein with 20-30% sequence identity to the known MAP kinase-regulated protein kinases RSK1/2/3, MNK1/2, and MAPKAP-K2/3. PRAK is activated by stress-related extra-cellular stimuli. The regulatory phosphorylation site of PRAK is Thr182, which is phosphorylated by p38 alpha and p38 beta in vitro. Activated PRAK specifically phosphorylates HSP27 at its physiologically relevant sites in vitro.
Recombinant full-length human PRAK with an N-terminal His-tag, expressed in Sf9 cells.
Mr ~54kD
Recombinant full-length human PRAK with an N-terminal His-tag, expressed in Sf9 cells.
Mr ~54kD
Product Categories/Family for PRAK, unactive active protein
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Product Notes
The PRAK, unactive (Catalog #AAA636621) is an Active Protein produced from Recombinant, human and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PRAK, unactive, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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