Testing Data
(Kinase Assay: 1-50ng of this lot of enzyme|phosphorylated 0.33mg/ml MBP in the assay|described on page two. Assay background was|subtracted from the actual counts to yield the results|shown below.)
SAPK2a, p38 alpha, active Active Protein
SAPK2a, p38 alpha, active (Stress-activated Protein Kinase 2a) Recombinant
Purity
Highly Purified98% by SDS and Coomassie Blue staining
Synonyms
SAPK2a; p38 alpha; active; active (Stress-activated Protein Kinase 2a) Recombinant; active active protein
Specificity
Essentially identical in mammals (p38 MAPK) and yeast (HOG-1). Inhibitors include protein phosphatase 2A, PTP-1B, CL-100 and pyridinyl imidazols.
Purity/Purification
Highly Purified
98% by SDS and Coomassie Blue staining
98% by SDS and Coomassie Blue staining
Form/Format
Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 50% glycerol.
Specific Activity
50units/mg using MBP as a substrate. One unit of SAPK2a/p38a = 1 nmol phosphate incorporated into MBP per minute.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Kinase Assay: 1-50ng of this lot of enzyme|phosphorylated 0.33mg/ml MBP in the assay|described on page two. Assay background was|subtracted from the actual counts to yield the results|shown below.)
Testing Data
(Kinase Assay: 1-50ng of this lot of enzyme|phosphorylated 0.33mg/ml MBP in the assay|described on page two. Assay background was|subtracted from the actual counts to yield the results|shown below.)
SDS-Page
(SDS-PAGE and Coomassie Stain: Representative gel from this lot.|Purity was assessed by SDS-PAGE and Coomassie blue staining using 3ug of S0096-28.)
SDS-Page
(SDS-PAGE and Coomassie Stain: Representative gel from this lot.|Purity was assessed by SDS-PAGE and Coomassie blue staining using 3ug of S0096-28.)
Related Product Information for SAPK2a, p38 alpha, active active protein
GST fusion protein containing full length human SAPK2a/p38a (64kDa).
Product Categories/Family for SAPK2a, p38 alpha, active active protein
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Product Notes
The SAPK2a, p38 alpha, active (Catalog #AAA635698) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SAPK2a, p38 alpha, active, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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