Typical Testing Data/Standard Curve (for reference only)
Mammalian N?-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit
N?-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit
Reactivity
Mammalian
Synonyms
N?-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit; N?-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit assay kit
Reactivity
Mammalian
Application Notes
Screening/studying/characterizing potential inhibitors of NNMT
Preparation and Storage
At -80 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for N?-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit assay kit
Background/Introduction: N?-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy. The NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using a Thiol Detecting Probe generating enhanced fluorescence signal that can be measured at Ex/Em = 392/482 nm. In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.
Product Categories/Family for N?-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit assay kit
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Product Notes
The Mammalian N?-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit (Catalog #AAA841838) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA841838 Assay Kit recognizes Mammalian N?-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit. Screening/studying/characterizing potential inhibitors of NNMT. Researchers should empirically determine the suitability of the N?-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N?-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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