Typical Testing Data/Standard Curve (for reference only)
pCAF assay kit
pCAF Inhibitor Screening Kit (Fluorometric)
Gene Names
KAT2B; PCAF
Synonyms
pCAF; pCAF Inhibitor Screening Kit (Fluorometric); P300/CBP-associated factor Inhibitor Screening Kit (Fluorometric); K (lysine) acetyltransferase 2B Inhibitor Screening Kit (Fluorometric); KAT2B Inhibitor Screening Kit (Fluorometric); pCAF assay kit
Sequence Length
760
Application Notes
Screening/studying/characterizing potential inhibitors of pCAF
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for pCAF assay kit
Background/Introduction: pCAF (P300/CBP-associated factor, EC 2.3.1.48) also known as K (lysine) acetyltransferase 2B (KAT2B), possesses intrinsic histone acetyltransferase (HAT) activity. pCAF contributes to transcriptional activation by acetylating chromatin and transcription factors through its HAT activity. Deregulated HAT activity plays an important role in various cancers. pCAF inhibitors have potential applications in cancer therapy. pCAF uses both histone 3 (H3) and histone 4 (H4) as substrates. pCAF Inhibitor Screening Kit utilizes H3 peptide and Acetyl CoA as the substrates. pCAF acetylates the peptide and generates Coenzyme A with a free thiol group. The CoA is detected using a Thiol Detecting Probe that reacts with thiol groups and gives enhanced fluorescence that can be measured at Ex/Em = 392/482 nm. In the presence of pCAF specific inhibitors, the enzymatic activity is inhibited resulting in decreased or total loss of fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential pCAF inhibitors.
Product Categories/Family for pCAF assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
87,461 Da
NCBI Official Full Name
PCAF, partial
NCBI Official Synonym Full Names
K(lysine) acetyltransferase 2B
NCBI Official Symbol
KAT2B
NCBI Official Synonym Symbols
PCAF
NCBI Protein Information
histone acetyltransferase KAT2B
UniProt Protein Name
PCAF
UniProt Gene Name
pcaf
UniProt Entry Name
Q90ZI1_CHICK
Research Articles on pCAF
Similar Products
Product Notes
The pCAF pcaf (Catalog #AAA841881) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Screening/studying/characterizing potential inhibitors of pCAF. Researchers should empirically determine the suitability of the pCAF pcaf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "pCAF, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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