Monoamine Oxidase A (MAO-A) Assay Kit | MAOA assay kit

Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric)

Cat. Num. AAA841864

• Synonyms: Monoamine Oxidase A (MAO-A); Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric); MAOA assay kit

• Sequence Length: 27

• Application Notes: Screening/studying/characterizing MAO-A inhibitors
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAOA assay kit

Background/Introduction: Monoamine oxidases (MAO, EC 1.4.3.4) are a family of enzymes that can oxidize a wide variety of endogenous primary amines. Two isoforms, MAO-A and MAO-B, have been identified based on their substrate, inhibitor specificity, and tissue localization. MAO-A can oxidize primary amines such as serotonin and norepinephrine. MAO-A is a mitochondrial-bound enzyme that is ubiquitously expressed throughout the brain and other tissues. It has been implicated in panic, anxiety, and depression. Several MAO-A specific inhibitors such as clorgyline, brofaromine, toloxatone, tetrindole, etc. have been used as antidepressants, but their usage has been limited due to side effects. The MAO-A Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-A inhibitors. The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of MAO substrate (Tyramine).

Product Categories/Family for MAOA assay kit

Neuroscience; ; Assay Kits; Metabolism Assays; Other Metabolism-Related Assays; Metabolism Assays; Metabolism Assays (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 4262061
• Molecular Weight: 44,848 Da
• NCBI Official Full Name: monoamine oxidase A, partial
• UniProt Protein Name: Amine oxidase [flavin-containing] A
• Protein Family: Amine oxidase
• UniProt Gene Name: MAOA
• UniProt Synonym Gene Names: MAO-A
• UniProt Entry Name: AOFA_HUMAN

NCBI Description

This gene is one of two neighboring gene family members that encode mitochondrial enzymes which catalyze the oxidative deamination of amines, such as dopamine, norepinephrine, and serotonin. Mutation of this gene results in Brunner syndrome. This gene has also been associated with a variety of other psychiatric disorders, including antisocial behavior. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

Uniprot Description

MAOA: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine. Defects in MAOA are the cause of Brunner syndrome (BRUNS). Brunner syndrome is a form of X-linked non- dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. Belongs to the flavin monoamine oxidase family.

Protein type: EC 1.4.3.4; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - tyrosine; Membrane protein, integral; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - histidine; Amino Acid Metabolism - tryptophan; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - glycine, serine and threonine; Oxidoreductase

Chromosomal Location of Human Ortholog: Xp11.3

Cellular Component: mitochondrial outer membrane; mitochondrion; integral to membrane

Molecular Function: amine oxidase activity

Biological Process: synaptic transmission; biogenic amine metabolic process; neurotransmitter catabolic process; dopamine catabolic process; neurotransmitter secretion; xenobiotic metabolic process; neurotransmitter biosynthetic process

Disease: Brunner Syndrome