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Typical Testing Data/Standard Curve (for reference only)

Branched Chain Amino Acid (Leu/Ile/Val) Assay Kit

Branched Chain Amino Acid (Leu / Ile / Val) Colorimetric Assay Kit

Synonyms
Branched Chain Amino Acid (Leu/Ile/Val); Branched Chain Amino Acid (Leu / Ile / Val) Colorimetric Assay Kit; Branched Chain Amino Acid (Leu/Ile/Val) assay kit
Ordering
For Research Use Only!
Samples
Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
Features & Benefits
Simple procedure; takes less than ~40 minutes
Fast and convenient
Preparation and Storage
At -20 degree C
Shelf Life: 12 months

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Branched Chain Amino Acid (Leu/Ile/Val) assay kit
Product Information: Branched Chain Amino Acid (Leu, Ile, Val) Assay Kit: Colorimetric Assay to Measure BCAA's in a variety of Biological Samples such as Tissue, Cells, Serum etc. within 40 min. Accurate, Convenient & Reliable. Detection limit: ~0.2 nmol. 100 Assays

Background: The branched-chain amino acids or BCAA's, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA's are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA's, primarily Leu, can stimulate insulin secretion. The BCAA's have also been implicated in a wide range of other physiological effects. MyBioSource's BCAA Assay Kit provides a simple convenient means of measuring the BCAA's in a variety of biological samples. The kit utilizes an enzyme assay in which BCAA is oxidatively deaminated, producing NADH which reduces the probe, generating a colored product (lambdamax = 450 nm). MyBioSource's BCAA kit measures BCAA's in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 uM BCAA in sample). BCAA's are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).

Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Applications- MyBioSource's BCAA kit measures BCAA's in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 uM BCAA in sample).
Product Categories/Family for Branched Chain Amino Acid (Leu/Ile/Val) assay kit

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Product Notes

The Branched Chain Amino Acid (Leu/Ile/Val) (Catalog #AAA841800) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Branched Chain Amino Acid (Leu/Ile/Val), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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