Typical Testing Data/Standard Curve (for reference only)
Coenzyme A (CoA) Assay Kit | Cob_03724 assay kit
Coenzyme A (CoA) Colorimetric / Fluorometric Assay Kit
Synonyms
Coenzyme A (CoA); Coenzyme A (CoA) Colorimetric / Fluorometric Assay Kit; Cob_03724 assay kit
Sequence Length
548
Samples
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Features & Benefits
Simple procedure; takes less than 1 hour to complete the assay
Fast and convenient
The assay is sensitive, stable and high-throughput adaptable
Fast and convenient
The assay is sensitive, stable and high-throughput adaptable
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Cob_03724 assay kit
Product Information: Coenzyme A Assay Kit: Measure CoA levels in Variety of Biological Samples. Easy, Accurate & Convenient Colorimetric Assay. Detection Limit: 0.1 to 10 nmol. 100 Assays.
Background: Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates. MyBioSource has developed an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (lambda = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 uM concentration range) in a variety of samples.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Applications- The assay can detect 0.1 to 10 nmol of CoA (2.5-250 uM concentration range) in a variety of samples.
Background: Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates. MyBioSource has developed an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (lambda = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 uM concentration range) in a variety of samples.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Applications- The assay can detect 0.1 to 10 nmol of CoA (2.5-250 uM concentration range) in a variety of samples.
Product Categories/Family for Cob_03724 assay kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
58,044 Da
NCBI Official Full Name
coenzyme a
UniProt Protein Name
Succinyl-CoA:3-ketoacid-coenzyme A transferase
UniProt Gene Name
Cob_03724
UniProt Entry Name
N4W3Z3_COLOR
Uniprot Description
Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
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Product Notes
The Coenzyme A (CoA) cob_03724 (Catalog #AAA841461) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Coenzyme A (CoA), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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