Human Elafin/PI3 ELISA Kit | PI3 elisa kit

Human Elafin/PI3 Quick ELISA Kit

Cat. Num. AAA1753946

• Gene Names: PI3; ESI; WAP3; SKALP; WFDC14; cementoin
• Reactivity: Human
• Synonyms: Elafin/PI3; Human Elafin/PI3 Quick ELISA Kit; Trappin-2/Elafin/Skalp|cementoin; Elafin; Elastase-specific inhibitor; ESI; ESIWAP four-disulfide core domain protein 14; Peptidase inhibitor 3; peptidase inhibitor 3; skin-derived; PI3; PI-3; pre-elafin; protease inhibitor 3; skin-derived (SKALP); Protease inhibitor WAP3; SKALP; SKALPELAFIN; Skin-derived antileukoproteinase; Trappin2; Trappin-2; WAP four-disulfide core domain 14; WAP3MGC13613; WFDC14elafin; PI3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human PI3

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 31.2pg/ml-2,000pg/ml
• Sensitivity: <10pg/ml
• Capture/Detection Antibodies: Capture antibody- polyclonal antibody from goat|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.024|0.154|0.244|0.461|0.821|1.425|2.122|2.274
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|67|269|902|; Standard deviation|4.36|12.91|50.51|; CV(%)|6.5%|4.8%|5.6%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|75|263|887; Standard deviation|5.33|14.73|60.32; CV(%)|7.1%|5.6%|6.8%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 67|55|59|53|59|3.80|6.5%; Sample 2: 269|260|270|259|265|11.37|4.3%; Sample 3: 902|996|955|1033|972|56.35|5.8%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A23-Q117
• Anticoagulant: heparin or EDTA
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human Elafin/PI3 with <10pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human PI3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human PI3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human PI3 with immunogen: Expression system for standard: NS0; Immunogen sequence: A23-Q117.; ; To measure Human PI3, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human PI3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human PI3 in the sample.
• Assay Procedure Step 1: 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.25pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for PI3 elisa kit

Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase(SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13. 12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core(WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Product Categories/Family for PI3 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 4505787
• NCBI GeneID: 5266
• NCBI Accession #: NP_002629.1
• NCBI GenBank Nucleotide #: NM_002638.3
• UniProt Accession #: P19957; Q6FG74; E1P618
• Molecular Weight: 12,270 Da
• NCBI Official Full Name: elafin preproprotein
• NCBI Official Synonym Full Names: peptidase inhibitor 3
• NCBI Official Symbol: PI3
• NCBI Official Synonym Symbols: ESI; WAP3; SKALP; WFDC14; cementoin
• NCBI Protein Information: elafin
• UniProt Protein Name: Elafin
• Protein Family: Elafin
• UniProt Gene Name: PI3
• UniProt Synonym Gene Names: WAP3; WFDC14; ESI; PI-3; SKALP
• UniProt Entry Name: ELAF_HUMAN

Uniprot Description

elafin: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Protein type: Extracellular matrix; Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: proteinaceous extracellular matrix

Molecular Function: endopeptidase inhibitor activity