Typical Testing Data/Standard Curve (for reference only)
Phosphate Assay Kit | EVA_18875 assay kit
Phosphate Colorimetric Assay Kit
Synonyms
Phosphate; Phosphate Colorimetric Assay Kit; EVA_18875 assay kit
Sequence Length
185
Samples
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids, algal blooms and water from run-off areas of high fertilizer use
Features & Benefits
Simple procedure; takes ~ 30 minutes
Fast and convenient
The assay is sensitive, stable and high-throughput adaptable
Fast and convenient
The assay is sensitive, stable and high-throughput adaptable
Preparation and Storage
Room Temperature
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for EVA_18875 assay kit
Product Information: Phosphate Colorimetric Assay Kit: Easy, Quick and Sensitive Assay to Measure Phosphate level within 30 min. Detection Limit: 0.1 nmol. 500 Assays.
Background: Phosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use. The newly designed Phosphate Colorimetric Assay Kit provides an easy, quick and sensitive means of assessing phosphate over a wide range of concentrations. The assay utilizes a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around 650 nm. Phosphate concentrations between 1 uM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined. The Phosphate Colorimetric Assay Kit provides 500 assays using microtiter plates or 100 assays using 1 ml cuvettes.
Summary: Detection method- Absorbance (650 nm)
Species reactivity- Mammalian
Applications- Phosphate concentrations between 1 uM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
Background: Phosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use. The newly designed Phosphate Colorimetric Assay Kit provides an easy, quick and sensitive means of assessing phosphate over a wide range of concentrations. The assay utilizes a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around 650 nm. Phosphate concentrations between 1 uM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined. The Phosphate Colorimetric Assay Kit provides 500 assays using microtiter plates or 100 assays using 1 ml cuvettes.
Summary: Detection method- Absorbance (650 nm)
Species reactivity- Mammalian
Applications- Phosphate concentrations between 1 uM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
Product Categories/Family for EVA_18875 assay kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
19,827 Da
NCBI Official Full Name
phosphate, partial
UniProt Protein Name
Phosphate
Protein Family
UniProt Gene Name
EVA_18875
UniProt Entry Name
J9FF27_9ZZZZ
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Product Notes
The Phosphate eva_18875 (Catalog #AAA841596) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Phosphate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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