Typical Testing Data/Standard Curve (for reference only)
Phosphate Assay Kit | EVA_18875 assay kit
Phosphate Fluorometric Assay Kit
Synonyms
Phosphate; Phosphate Fluorometric Assay Kit; EVA_18875 assay kit
Sequence Length
185
Samples
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids, algal blooms and water from run-off areas of high fertilizer use
Features & Benefits
Simple procedure; takes only ~2 hours
Fast and convenient
The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5'-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase, etc. from a variety of samples.
Fast and convenient
The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5'-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase, etc. from a variety of samples.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for EVA_18875 assay kit
Product Information: Phosphate Fluorometric Assay Kit: Easy, Quick and Sensitive Assay to Measure Phosphate level. Detection Limit~ 40 pmol. 100 Assays.
Background: Inorganic phosphate (Pi) is one of the most important ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. A highly sensitive assay is desired to monitor Pi in variety samples or monitor Pi changes during kinase and phosphatase reactions. The newly designed Phosphate Assay Kit provides a sensitive, easy, quick means of assessing phosphate over a wide range of concentrations. In the assay, inorganic phosphate reacted with maltose to produce glucose in the present of a special enzyme. The glucose is specifically oxidized to generate a product that reacts with the OxiRed probe to generate fluorescence (Ex/Em=535/587nm). The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5'-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase, etc. from a variety of samples. Glucose background can be eliminated by doing a glucose control. Phosphate concentrations between 0.4 uM and 10 uM, with a lower detection limit of approximately 40 pmol can be directly determined.
Summary: Detection method- Fluorescence (Ex/Em 535/587nm)
Species reactivity- Mammalian
Applications- Phosphate concentrations between 0.4 uM and 10 uM, with a lower detection limit of approximately 40 pmol can be directly determined.
Background: Inorganic phosphate (Pi) is one of the most important ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. A highly sensitive assay is desired to monitor Pi in variety samples or monitor Pi changes during kinase and phosphatase reactions. The newly designed Phosphate Assay Kit provides a sensitive, easy, quick means of assessing phosphate over a wide range of concentrations. In the assay, inorganic phosphate reacted with maltose to produce glucose in the present of a special enzyme. The glucose is specifically oxidized to generate a product that reacts with the OxiRed probe to generate fluorescence (Ex/Em=535/587nm). The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5'-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase, etc. from a variety of samples. Glucose background can be eliminated by doing a glucose control. Phosphate concentrations between 0.4 uM and 10 uM, with a lower detection limit of approximately 40 pmol can be directly determined.
Summary: Detection method- Fluorescence (Ex/Em 535/587nm)
Species reactivity- Mammalian
Applications- Phosphate concentrations between 0.4 uM and 10 uM, with a lower detection limit of approximately 40 pmol can be directly determined.
Product Categories/Family for EVA_18875 assay kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
19,827 Da
NCBI Official Full Name
phosphate, partial
UniProt Protein Name
Phosphate
Protein Family
UniProt Gene Name
EVA_18875
UniProt Entry Name
J9FF27_9ZZZZ
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Product Notes
The Phosphate eva_18875 (Catalog #AAA841464) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Phosphate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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