Typical Testing Data/Standard Curve (for reference only)
Chloride Assay Kit
Chloride Colorimetric Assay Kit
Synonyms
Chloride; Chloride Colorimetric Assay Kit; Chloride assay kit
Samples
Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
Features & Benefits
Simple procedure; takes less than ~20 minutes
Fast and convenient•Mn, Cu, Zn do not interfere with the assay.
Fast and convenient•Mn, Cu, Zn do not interfere with the assay.
Preparation and Storage
Room Temperature
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Chloride assay kit
Product Information: Chloride Assay Kit: Colorimetric Assay to quantitate Chloride in a variety of biological samples such as Blood, Urine etc. within 20 min. Detection Sensitivity ~0.4 mM. 100 Assays.
Background: Chloride is the anionic form of chlorine. It is the most common of the anions found in living organisms. Chloride ions play a variety of important physiological roles. Chloride channels are found in a variety of cells and are responsible for setting resting cell membrane potential and regulating cell volume. In the nervous system, the action of glycine and GABA are related to chloride levels in specific neurons. Chloride is also instrumental in maintaining the acid-base balance in blood. The kidneys are instrumental in closely regulating serum chloride levels. There are a number of pathologies associated with defective chloride transport; the most well-known being Cystic Fibrosis, caused by a mutation in CFTR a membrane chloride transporter. MyBioSource's Chloride Assay Kit provides a quick, simple method for quantification of Chloride in a variety of biological samples. Blood and urine can be used directly after dilution with water. The assay is based upon the competition of Hg and Fe for TPTZ. The preferred Hg-TPTZ adduct exhibits no color. In the presence of Chloride, Hg forms HgCL2 freeing up TPTZ which then binds the available Fe giving a very intense absorbance with a lambdamax~ 620nm. The assay is linear in the range 20 to 120 nmol Chloride/well with detection sensitivity ~0.4 mM chloride.
Summary: Detection method- Absorbance (620 nm)
Species reactivity- Mammalian
Applications- The assay is linear in the range 20 to 120 nmol Chloride/well with detection sensitivity ~0.4 mM chloride.
Background: Chloride is the anionic form of chlorine. It is the most common of the anions found in living organisms. Chloride ions play a variety of important physiological roles. Chloride channels are found in a variety of cells and are responsible for setting resting cell membrane potential and regulating cell volume. In the nervous system, the action of glycine and GABA are related to chloride levels in specific neurons. Chloride is also instrumental in maintaining the acid-base balance in blood. The kidneys are instrumental in closely regulating serum chloride levels. There are a number of pathologies associated with defective chloride transport; the most well-known being Cystic Fibrosis, caused by a mutation in CFTR a membrane chloride transporter. MyBioSource's Chloride Assay Kit provides a quick, simple method for quantification of Chloride in a variety of biological samples. Blood and urine can be used directly after dilution with water. The assay is based upon the competition of Hg and Fe for TPTZ. The preferred Hg-TPTZ adduct exhibits no color. In the presence of Chloride, Hg forms HgCL2 freeing up TPTZ which then binds the available Fe giving a very intense absorbance with a lambdamax~ 620nm. The assay is linear in the range 20 to 120 nmol Chloride/well with detection sensitivity ~0.4 mM chloride.
Summary: Detection method- Absorbance (620 nm)
Species reactivity- Mammalian
Applications- The assay is linear in the range 20 to 120 nmol Chloride/well with detection sensitivity ~0.4 mM chloride.
Product Categories/Family for Chloride assay kit
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Product Notes
The Chloride (Catalog #AAA841693) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Chloride, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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