CLEC4F recombinant protein

CLEC4F Protein, Rat, Recombinant

Cat. Num. AAA8123381

• Gene Names: Clec4f; Kclr; Clecsf13
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CLEC4F; CLEC4F Protein; Rat; Recombinant; Rat CLEC4F/CLECSF13 Protein; C-type lectin domain family 4; member F; CLEC4F recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Arg70-Ser550

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the rat CLEC4F(NP_446205.1)(Arg70-Ser550) was expressed with two additional amino acids (Gly & Pro) at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CLEC4F recombinant protein

Background: CLEC4F, a member of C-type lectins, was firstly purified from rat liver extract with high binding affinity to fucose, galactose and N-acetylgalactosamine, and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated. Histochemical staining showed that mouse CLEC4F(mCLEC4F) is only expressed on F4/8+ cells localized in liver, and is undetectable in bone marrow, spleen, lymph nodes, or other tissues in adult mice. However, mCLEC4F is detected in the liver of embryonic day 11.5 (E11.5), which is 1.5 day earlier than the formation of liver (E1) and is 3.5 day earlier than the formation of bone marrow (E15-16). Moreover, recombinant mCLEC4F.Fc binds to alpha-galactoceramide in a Ca++-dependent manner, and both galactose and ceramide can partially inhibit CLEC4F.Fc binding to alpha-galactoceramide. Interestingly, mCLEC4F-deficient (mCEC4F k/o) mice produced far less cytokines than wild type littermates after intravenous injection ofalpha-galactoceramide. This suggests that mCLEC4F is not only a specific marker for Kupffer cells, but is also critical for the presentation of glycolipid antigen to NKT cells.

References

Shie-Liang Edmond Hsieh, et al. (2009) CLEC4F, A Kupffer Cells Specific Marker, Is Critical for Presentation of Alfa-Galactoceromide to NKT Cells. The Journal of Immunology. 182:78. Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Bonaldo MF, et al. (1996) Normalization and subtraction: two approaches to facilitate gene discovery. Genome Res. 1996 Sep;6(9):791-806.

NCBI and Uniprot Product Information

• NCBI GI #: 16758588
• NCBI GeneID: 114598
• NCBI Accession #: NP_446205.1
• NCBI GenBank Nucleotide #: NM_053753.1
• UniProt Accession #: P10716; Q4KMB0
• Molecular Weight: 61,104 Da
• NCBI Official Full Name: C-type lectin domain family 4 member F
• NCBI Official Synonym Full Names: C-type lectin domain family 4, member F
• NCBI Official Symbol: Clec4f
• NCBI Official Synonym Symbols: Kclr; Clecsf13
• NCBI Protein Information: C-type lectin domain family 4 member F; C-type lectin 13; Kupffer cell receptor; C-type lectin superfamily member 13; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 13
• UniProt Protein Name: C-type lectin domain family 4 member F
• Protein Family: C-type lectin domain family
• UniProt Gene Name: Clec4f
• UniProt Synonym Gene Names: Clecsf13; Kclr; C-type lectin 13
• UniProt Entry Name: CLC4F_RAT