Smad5 recombinant protein

Smad5 Protein, Mouse, Recombinant

Cat. Num. AAA8122956

• Gene Names: Smad5; Dwf-C; Madh5; MusMLP; AI451355; 1110051M15Rik
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Smad5; Smad5 Protein; Mouse; Recombinant; Mouse Smad5 Protein; 1110051M15Rik Protein; AI451355 Protein; Dwf-C Protein; Madh5 Protein; MusMLP Protein; SMAD family member 5; Smad5 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM Nacl,10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Thr2-Ser465

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the mouse SMAD5 (P97454)(Thr2-Ser465) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for Smad5 recombinant protein

Background: SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

Product Categories/Family for Smad5 recombinant protein

TGF-beta Signaling

References

Vinayagam A. et al., 2011, Sci Signal. 4 (189): rs8. Sangadala S. et al., 2007, J Biomol Struct Dyn. 25 (1): 11-23. Riggins GJ. et al., 1996, Nat Genet. 13 (3): 347-9.

NCBI and Uniprot Product Information

• NCBI GI #: 255708405
• NCBI GeneID: 17129
• NCBI Accession #: NP_001157513.1
• NCBI GenBank Nucleotide #: NM_001164041.1
• UniProt Accession #: P97454; P70341; Q810K0
• Molecular Weight: 52,172 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 5
• NCBI Official Synonym Full Names: SMAD family member 5
• NCBI Official Symbol: Smad5
• NCBI Official Synonym Symbols: Dwf-C; Madh5; MusMLP; AI451355; 1110051M15Rik
• NCBI Protein Information: mothers against decapentaplegic homolog 5; Smad 5; mSmad5; dwarfin-C; MAD homolog 5; mothers against DPP homolog 5
• UniProt Protein Name: Mothers against decapentaplegic homolog 5
• Protein Family: SMAD5 antisense gene protein
• UniProt Gene Name: Smad5
• UniProt Synonym Gene Names: Madh5; Msmad5; MAD homolog 5; Mothers against DPP homolog 5; Dwf-C; SMAD 5; Smad5; mSmad5
• UniProt Entry Name: SMAD5_MOUSE