Rabbit anti-Human, Mouse UBE1C Polyclonal Antibody | anti-UBE1C antibody

UBE1C, CT (Ubiquitin-activating Enzyme E1C, NEDD8-activating Enzyme E1 Catalytic Subunit, Ubiquitin-like Modifier-activating Enzyme 3, Ubiquitin-activating Enzyme 3, NEDD8-activating Enzyme E1C, UBA3) (PE)

Cat. Num. AAA6504451

• Gene Names: UBA3; NAE2; UBE1C; hUBA3
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: UBE1C; Polyclonal Antibody; CT (Ubiquitin-activating Enzyme E1C; NEDD8-activating Enzyme E1 Catalytic Subunit; Ubiquitin-like Modifier-activating Enzyme 3; Ubiquitin-activating Enzyme 3; NEDD8-activating Enzyme E1C; UBA3) (PE); anti-UBE1C antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UBE1C. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-UBE1C antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human UBE1C (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(The anti-UBE1 C-term Pab is used in Western blot to detect UBE1 in mouse brain tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(UBE1C Antibody (S430) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the UBE1C antibody detected the UBE1C protein (arrow).)

Western Blot (WB)

(Western blot analysis of UBE1C (arrow) using rabbit polyclonal UBE1C Antibody (S430). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE1C gene.)

Product Categories/Family for anti-UBE1C antibody

Antibodies; Ubiquitin

References

1. Bohnsack, R.N., et al., J. Biol. Chem. 278(29):26823-26830 (2003). 2. Walden, H., et al., Nature 422(6929):330-334 (2003). 3. Gong, L., et al., J. Biol. Chem. 274(17):12036-12042 (1999). 4. Gubin, A.N., et al., Genomics 59(2):168-177 (1999). 5. Osaka, F., et al., Genes Dev. 12(15):2263-2268 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 38045942
• NCBI GeneID: 9039
• NCBI Accession #: NP_003959
• NCBI GenBank Nucleotide #: NM_003968
• UniProt Accession #: Q8TBC4; O76088; Q9NTU3; A6NLB5; A8K027
• Molecular Weight: 50,072 Da
• NCBI Official Full Name: NEDD8-activating enzyme E1 catalytic subunit isoform 1
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 3
• NCBI Official Symbol: UBA3
• NCBI Official Synonym Symbols: NAE2; UBE1C; hUBA3
• NCBI Protein Information: NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1 subunit 2; NEDD8-activating enzyme E1C; Nedd8-activating enzyme hUba3; UBA3, ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme 3; ubiquitin-activating enzyme E1C (U
• UniProt Protein Name: NEDD8-activating enzyme E1 catalytic subunit
• UniProt Gene Name: UBA3
• UniProt Synonym Gene Names: UBE1C; Ubiquitin-activating enzyme 3
• UniProt Entry Name: UBA3_HUMAN

Uniprot Description

UBA3: Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; EC 6.3.2.19; Ubiquitin ligase; Ligase; Ubiquitin conjugating system; Cell cycle regulation; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: nucleus; cytosol

Molecular Function: NEDD8 activating enzyme activity; protein binding; acid-amino acid ligase activity; protein heterodimerization activity; ATP binding

Biological Process: protein neddylation; regulation of cell cycle; protein modification process; endomitotic cell cycle; proteolysis