NRG4 recombinant protein

NRG4 Protein, Human, Recombinant

Cat. Num. AAA8121113

• Gene Names: NRG4; HRG4
• Purity: >92% as determined by SDS-PAGE
• Synonyms: NRG4; NRG4 Protein; Human; Recombinant; Human NRG4 Protein; HRG4 Protein; neuregulin 4; NRG4 recombinant protein

• Host: E Coli
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 50mM NaCl, pH7.2. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Pro2-Phe62

• Species: Human
• Predicted N Terminal: Gly
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the human NRG4 (Q8WWG1) extracellular domain (Pro 2-Phe 62) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NRG4 recombinant protein

Background: NRG4 (neuregulin 4) is a member of the neuregulin protein family. The neuregulins consist of four structurally-related proteins that are part of the EGF family of proteins. It has been shown that these proteins have diverse functions in the development of the nervous system and play multiple essential roles in vertebrate embryogenesis including cardiac development, Schwann cell, and oligodendrocyte differentiation, some aspects of neuronal development, as well as the formation of neuromuscular synapses. NRG4 contains 1 EGF-like domain. It activates type-1 growth factor receptors to initiating cell-to-cell signaling through tyrosine phosphorylation. NRG4 is a low-affinity ligand for the ERBB4 tyrosine kinase receptor. It concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. However, it does not bind to the ERBB1, ERBB2, and ERBB3 receptors.

References

Strausberg RL, et al. (2002) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Nat Acad Sci. 99(26):16899-903.Harari D, et al. (1999) Neuregulin-4: a novel growth factor that acts through the ErbB-4 receptor tyrosine kinase. Oncogene. 18(17):2681-9.Memon AA, et al. (2004) Expression of HER3, HER4 and their ligand heregulin-4 is associated with better survival in bladder cancer patients. Br J Cancer. 91(12) 2034-41.

NCBI and Uniprot Product Information

• NCBI GI #: 28201832
• NCBI GeneID: 145957
• UniProt Accession #: Q8WWG1; A6NIE8
• NCBI Official Full Name: Pro-neuregulin-4, membrane-bound isoform
• NCBI Official Synonym Full Names: neuregulin 4
• NCBI Official Symbol: NRG4
• NCBI Official Synonym Symbols: HRG4
• NCBI Protein Information: pro-neuregulin-4, membrane-bound isoform; pro-NRG4; heregulin 4
• UniProt Protein Name: Pro-neuregulin-4, membrane-bound isoform
• Protein Family: Pro-neuregulin
• UniProt Gene Name: NRG4
• UniProt Synonym Gene Names: Pro-NRG4; NRG-4
• UniProt Entry Name: NRG4_HUMAN

NCBI Description

The neuregulins, including NRG4, activate type-1 growth factor receptors (see EGFR; MIM 131550) to initiating cell-to-cell signaling through tyrosine phosphorylation (Harari et al., 1999 [PubMed 10348342]).[supplied by OMIM, Mar 2008]

Uniprot Description

NRG4: Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors. Belongs to the neuregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: extracellular space; integral to membrane; extracellular region; plasma membrane

Molecular Function: growth factor activity; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; organ development; innate immune response