Rabbit anti-Human CRISP1 Polyclonal Antibody | anti-CRISP1 antibody

CRISP1, CT (CRISP1, AEGL1, Cysteine-rich secretory protein 1, AEG-like protein, ARP, Acidic epididymal glycoprotein homolog) (Azide free) (HRP)

Cat. Num. AAA6288850

• Gene Names: CRISP1; ARP; AEGL1; HUMARP; CRISP-1; HSCRISP1D; HSCRISP1G
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CRISP1; Polyclonal Antibody; CT (CRISP1; AEGL1; Cysteine-rich secretory protein 1; AEG-like protein; ARP; Acidic epididymal glycoprotein homolog) (Azide free) (HRP); Acidic epididymal glycoprotein homolog; anti-CRISP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CRISP1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CRISP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 162-191 in the C-terminal region of human CRISP1, UniProt Accession #NP_001122.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB231 cell line lysates using MBS6000123 (35ug/lane).)

Related Product Information for anti-CRISP1 antibody

Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. The protein encoded by this gene is a member of the cysteine-rich secretory protein (CRISP) family. This protein is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head where it plays a role at fertilization in sperm-egg fusion through complementary sites localized on the egg surface. Two isoforms are encoded by transcript variants of this gene.

Product Categories/Family for anti-CRISP1 antibody

Antibodies; Amino Acids

References

1. Mungall, A.J., et al., Nature 425(6960): 805-811 (2003). 2. Evans, J.P., Hum. Reprod. Update 8(4): 297-311 (2002). 3. Cuasnicu, P.S., et al., Arch. Med. Res. 32(6): 614-618 (2001). 4. Cohen, D.J., et al., Biol. Reprod. 65(4): 1000-1005 (2001). 5. Kirchhoff, C., Rev. Reprod. 3(2): 86-95 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 25121982
• NCBI GeneID: 167
• NCBI Accession #: NP_001122.2; NP_733758.1
• NCBI GenBank Nucleotide #: NM_001131.2
• UniProt Accession #: P54107; O00698; Q13248; Q14082; Q96SF6; B5BU98
• Molecular Weight: 20,462 Da
• NCBI Official Full Name: cysteine-rich secretory protein 1 isoform 1
• NCBI Official Synonym Full Names: cysteine-rich secretory protein 1
• NCBI Official Symbol: CRISP1
• NCBI Official Synonym Symbols: ARP; AEGL1; HUMARP; CRISP-1; HSCRISP1D; HSCRISP1G
• NCBI Protein Information: cysteine-rich secretory protein 1; AEG-like protein; AEG-related protein; acidic epididymal glycoprotein homolog; acidic epididymal glycoprotein-like 1; cysteine-rich secretory protein-1 delta
• UniProt Protein Name: Cysteine-rich secretory protein 1
• Protein Family: Cysteine-rich secretory protein
• UniProt Gene Name: CRISP1
• UniProt Synonym Gene Names: AEGL1; CRISP-1
• UniProt Entry Name: CRIS1_HUMAN

NCBI Description

Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. The protein encoded by this gene is a member of the cysteine-rich secretory protein (CRISP) family. It is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head, where it plays a role in sperm-egg fusion. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

CRISP1: May have a role in sperm-egg fusion and maturation. Belongs to the CRISP family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; nucleus

Molecular Function: calcium channel regulator activity

Biological Process: fusion of sperm to egg plasma membrane; binding of sperm to zona pellucida; regulation of acrosome reaction