SDS-PAGE
CLEC4F / CLECSF13 recombinant protein
Recombinant Rat CLEC4F / CLECSF13 Protein
Gene Names
Clec4f; Kclr; Clecsf13
Purity
> 95 % as determined by SDS-PAGE
Synonyms
CLEC4F / CLECSF13; Recombinant Rat CLEC4F / CLECSF13 Protein; CLEC4F; Clecsf13; Kclr; CLEC4F / CLECSF13 recombinant protein
Host
Human Cells
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
550
Application Notes
The recombinant rat CLEC4F comprises 483 amino acids and predicts a molecular mass of 53.7 kDa. The apparent molecular mass of the recombinant protein is approximately 63-68 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Predicted N Terminal
Gly
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for CLEC4F / CLECSF13 recombinant protein
Background: CLEC4F, a member of C-type lectins, was firstly purified from rat liver extract with high binding affinity to fucose, galactose and N-acetylgalactosamine, and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated. Histochemical staining showed that mouse CLEC4F (mCLEC4F) is only expressed on F4/80+ cells localized in liver, and is undetectable in bone marrow, spleen, lymph nodes, or other tissues in adult mice. However, mCLEC4F is detected in the liver of embryonic day 11.5 (E11.5), which is 1.5 day earlier than the formation of liver (E10) and is 3.5 day earlier than the formation of bone marrow (E15-16). Moreover, recombinant mCLEC4F.Fc binds to alpha-galactoceramide in a Ca++-dependent manner, and both galactose and ceramide can partially inhibit CLEC4F.Fc binding to alpha-galactoceramide. Interestingly, mCLEC4F-deficient (mCEC4F k/o) mice produced far less cytokines than wild type littermates after intravenous injection ofalpha-galactoceramide. This suggests that mCLEC4F is not only a specific marker for Kupffer cells, but is also critical for the presentation of glycolipid antigen to NKT cells.
Description: A DNA sequence encoding the rat CLEC4F (NP_446205.1) (Arg70-Ser550) was expressed with two additional amino acids (Gly & Pro) at the N-terminus.
Description: A DNA sequence encoding the rat CLEC4F (NP_446205.1) (Arg70-Ser550) was expressed with two additional amino acids (Gly & Pro) at the N-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,104 Da
NCBI Official Full Name
C-type lectin domain family 4 member F
NCBI Official Synonym Full Names
C-type lectin domain family 4, member F
NCBI Official Symbol
Clec4f
NCBI Official Synonym Symbols
Kclr; Clecsf13
NCBI Protein Information
C-type lectin domain family 4 member F
UniProt Protein Name
C-type lectin domain family 4 member F
UniProt Gene Name
Clec4f
UniProt Synonym Gene Names
Clecsf13; Kclr; C-type lectin 13
NCBI Description
carbohydrate binding receptor with an affinity for fucose and galactose [RGD, Feb 2006]
Uniprot Description
Receptor with an affinity for galactose and fucose. Could be involved in endocytosis.
Research Articles on CLEC4F / CLECSF13
Similar Products
Product Notes
The CLEC4F / CLECSF13 clec4f (Catalog #AAA2547419) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant rat CLEC4F comprises 483 amino acids and predicts a molecular mass of 53.7 kDa. The apparent molecular mass of the recombinant protein is approximately 63-68 kDa in SDS-PAGE under reducing conditions due to glycosylation. Researchers should empirically determine the suitability of the CLEC4F / CLECSF13 clec4f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLEC4F / CLECSF13, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
