Rabbit anti-Rat NCAM Polyclonal Antibody | anti-NCAM1 antibody

Anti-NCAM Antibody, Rabbit Polyclonal

Cat. Num. AAA8112995

• Gene Names: Ncam1; Cd56; Ncam; N-CAM; NCAMC; NCAM-1; NCAM-C; N-CAM-1
• Reactivity: Rat
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: NCAM; Polyclonal Antibody; Anti-NCAM Antibody; Rabbit Polyclonal; NCAM1/CD56 Antibody; Rabbit PAb; Antigen Affinity Purified; neural cell adhesion molecule 1; anti-NCAM1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat NCAM1/CD56
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NCAM1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Rat NCAM1/CD56 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rat NCAM1/CD56 (rR NCAM1/CD56). NCAM1/CD56 specific IgG was purified by Rat NCAM1/CD56 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NCAM1 rabbit polyclonal antibody at 1:500 dilution Lane A: SHSY5Y Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:93 kDa Observed band size:200 kDa)

Related Product Information for anti-NCAM1 antibody

NCAM1 (Neural Cell Adhesion Molecule 1, also known as CD56) is a Protein Coding gene. 3 alternatively spliced human isoforms have been reported. NCAM1 is a neural adhesion protein (NCAM) that belongs to the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. NCAM1 is involved in neuron-neuron adhesion, neurite fasciculation, the outgrowth of neurites, etc. It has also been shown to be involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Diseases associated with NCAM1 include Rabies and Bile Duct Cancer. Among its related pathways are Neuroscience and RET signaling.

References

Reyes AA. et al., 1991, Mol Cell Biol. 11 (3): 1654-61. Suzuki M. et al., 2003, J Biol Chem. 278 (49): 49459-68. Becker C G. et al., 1996, J Neurosci Res. 45 (2): 143-52.

NCBI and Uniprot Product Information

• NCBI GI #: 13928706
• NCBI GeneID: 24586
• NCBI Accession #: NP_113709.1
• NCBI GenBank Nucleotide #: NM_031521.1
• UniProt Accession #: P13596
• Molecular Weight: 94,658 Da
• NCBI Official Full Name: neural cell adhesion molecule 1
• NCBI Official Synonym Full Names: neural cell adhesion molecule 1
• NCBI Official Symbol: Ncam1
• NCBI Official Synonym Symbols: Cd56; Ncam; N-CAM; NCAMC; NCAM-1; NCAM-C; N-CAM-1
• NCBI Protein Information: neural cell adhesion molecule 1
• UniProt Protein Name: Neural cell adhesion molecule 1
• Protein Family: Neural cell adhesion molecule
• UniProt Gene Name: Ncam1
• UniProt Synonym Gene Names: Ncam; N-CAM-1; NCAM-1

NCBI Description

cell adhesion molecule; involved in neuronal cell adhesion [RGD, Feb 2006]

Uniprot Description

NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8q23

Cellular Component: axon; cell soma; cell surface; cytoplasm; external side of plasma membrane; growth cone; intercellular junction; membrane; myelin sheath; plasma membrane

Molecular Function: cytoskeletal protein binding; fibroblast growth factor receptor binding; identical protein binding; LRR domain binding; phosphatase binding; protein binding

Biological Process: aging; axon guidance; axon regeneration in the peripheral nervous system; calcium-independent cell-cell adhesion; cell adhesion; cell surface receptor linked signal transduction; homotypic cell-cell adhesion; learning and/or memory; multicellular organismal response to stress; neurite development; neuron development; organ regeneration; positive regulation of calcium-mediated signaling; positive regulation of cardiac muscle cell proliferation; regulation of exocyst assembly; regulation of sensory perception of pain; response to cocaine; response to drug; response to inorganic substance; response to lead ion; thalamus development