Mouse anti-Human NCAM Monoclonal Antibody | anti-NCAM1 antibody

Anti-NCAM Antibody, Mouse Monoclonal

Cat. Num. AAA8103479

• Gene Names: NCAM1; CD56; NCAM; MSK39
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: NCAM; Monoclonal Antibody; Anti-NCAM Antibody; Mouse Monoclonal; NCAM1/CD56 Antibody; Mouse MAb; neural cell adhesion molecule 1; Anti-CD56 Antibody; Anti-MSK39 Antibody; anti-NCAM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 05
• Specificity: Human NCAM1/CD56
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NCAM1 antibody: ELISA (EIA), Flow Cytometry (FC/FACS/FCM)
• Application Notes: ELISA: 1:1000-1:2000; FC/FACS/FCM: 1:25-1:100
• Immunogen: Recombinant Human NCAM1/CD56 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human NCAM1/CD56 (rh NCAM1/CD56). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human NCAM1(CD56) expression on human whole blood lymphocyte. Cells were stained with purified anti-Human NCAM1(CD56), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)

Related Product Information for anti-NCAM1 antibody

NCAM1 (Neural Cell Adhesion Molecule 1, also known as CD56) is a Protein Coding gene. 3 alternatively spliced human isoforms have been reported. NCAM1 is a neural adhesion protein (NCAM) that belongs to the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. NCAM1 is involved in neuron-neuron adhesion, neurite fasciculation, the outgrowth of neurites, etc. It has also been shown to be involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Diseases associated with NCAM1 include Rabies and Bile Duct Cancer. Among its related pathways are Neuroscience and RET signaling.

References

Reyes AA. et al., 1991, Mol Cell Biol. 11 (3): 1654-61. Suzuki M. et al., 2003, J Biol Chem. 278 (49): 49459-68. Becker C G. et al., 1996, J Neurosci Res. 45 (2): 143-52.

NCBI and Uniprot Product Information

• NCBI GI #: 115529478
• NCBI GeneID: 4684
• NCBI Accession #: NP_001070150.1
• NCBI GenBank Nucleotide #: NM_001076682.3
• UniProt Accession #: P13591; P13592; P13593; Q05C58; Q15829; Q16180; Q16209; Q59FL7; Q86X47; Q96CJ3; A8K8T8
• Molecular Weight: Molecular Mass: 66.1 kDa; Actual Protein Molecular Mass: 66.1 kDa
• NCBI Official Full Name: neural cell adhesion molecule 1 isoform 3
• NCBI Official Synonym Full Names: neural cell adhesion molecule 1
• NCBI Official Symbol: NCAM1
• NCBI Official Synonym Symbols: CD56; NCAM; MSK39
• NCBI Protein Information: neural cell adhesion molecule 1
• UniProt Protein Name: Neural cell adhesion molecule 1
• Protein Family: Neural cell adhesion molecule
• UniProt Gene Name: NCAM1
• UniProt Synonym Gene Names: NCAM; N-CAM-1; NCAM-1
• UniProt Entry Name: NCAM1_HUMAN

NCBI Description

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

Uniprot Description

NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: Golgi membrane; cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: identical protein binding

Biological Process: axon guidance; extracellular matrix organization and biogenesis; cytokine and chemokine mediated signaling pathway; cell adhesion