Rabbit anti-Human NCAM Polyclonal Antibody | anti-NCAM1 antibody

Anti-NCAM Antibody, Rabbit Polyclonal

Cat. Num. AAA8103487

• Gene Names: NCAM1; CD56; NCAM; MSK39
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: NCAM; Polyclonal Antibody; Anti-NCAM Antibody; Rabbit Polyclonal; NCAM1/CD56 Antibody; Rabbit PAb; Antigen Affinity Purified; neural cell adhesion molecule 1; Anti-CD56 Antibody; Anti-MSK39 Antibody; anti-NCAM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NCAM1/CD56
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NCAM1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 0.5-2uL/mg of lysate
• Immunogen: Recombinant Human NCAM1/CD56 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human NCAM1/CD56 (rh NCAM1/CD56). NCAM1/CD56 specific IgG was purified by Human NCAM1/CD56 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NCAM1 rabbit polyclonal antibody at 1:500 dilution.Lane A: SH-SY5Y Whole Cell LysateLane B: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:95 kDaObserved band size:180 kDa(We are unsure as to the identity of these extra bands.))

Immunoprecipitation (IP)

(NCAM1 was immunoprecipitated using:Lane A:0.5 mg SH-SY5Y Whole Cell Lysate2 uL anti-NCAM1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NCAM1 rabbit polyclonal antibody,at 1:70 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 95 kDaObserved band size: 170 kDa)

Related Product Information for anti-NCAM1 antibody

NCAM1 (Neural Cell Adhesion Molecule 1, also known as CD56) is a Protein Coding gene. 3 alternatively spliced human isoforms have been reported. NCAM1 is a neural adhesion protein (NCAM) that belongs to the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. NCAM1 is involved in neuron-neuron adhesion, neurite fasciculation, the outgrowth of neurites, etc. It has also been shown to be involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Diseases associated with NCAM1 include Rabies and Bile Duct Cancer. Among its related pathways are Neuroscience and RET signaling.

References

Reyes AA. et al., 1991, Mol Cell Biol. 11 (3): 1654-61. Suzuki M. et al., 2003, J Biol Chem. 278 (49): 49459-68. Becker C G. et al., 1996, J Neurosci Res. 45 (2): 143-52.

NCBI and Uniprot Product Information

• NCBI GI #: 115529478
• NCBI GeneID: 4684
• NCBI Accession #: NP_001070150.1
• NCBI GenBank Nucleotide #: NM_001076682.3
• UniProt Accession #: P13591; P13592; P13593; Q05C58; Q15829; Q16180; Q16209; Q59FL7; Q86X47; Q96CJ3; A8K8T8
• Molecular Weight: Molecular Mass: 66.1 kDa; Actual Protein Molecular Mass: 66.1 kDa
• NCBI Official Full Name: neural cell adhesion molecule 1 isoform 3
• NCBI Official Synonym Full Names: neural cell adhesion molecule 1
• NCBI Official Symbol: NCAM1
• NCBI Official Synonym Symbols: CD56; NCAM; MSK39
• NCBI Protein Information: neural cell adhesion molecule 1
• UniProt Protein Name: Neural cell adhesion molecule 1
• Protein Family: Neural cell adhesion molecule
• UniProt Gene Name: NCAM1
• UniProt Synonym Gene Names: NCAM; N-CAM-1; NCAM-1
• UniProt Entry Name: NCAM1_HUMAN

NCBI Description

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

Uniprot Description

NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: Golgi membrane; cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: identical protein binding

Biological Process: axon guidance; extracellular matrix organization and biogenesis; cytokine and chemokine mediated signaling pathway; cell adhesion