NFkB Activating Kinase, active Active Protein
NFkB Activating Kinase, active, Recombinant, Human (Nuclear Factor kappa B, TANK-Binding Kinase 1, NAK, TBK1)
Purity
PurifiedPurified using Ni2+/NTA agarose. ~42% by SDS PAGE and Coomassie blue staining.
Synonyms
NFkB Activating Kinase; active; Recombinant; Human (Nuclear Factor kappa B; TANK-Binding Kinase 1; NAK; TBK1); active active protein
Host
Sf21 cells
Purity/Purification
Purified
Purified using Ni2+/NTA agarose. ~42% by SDS PAGE and Coomassie blue staining.
Purified using Ni2+/NTA agarose. ~42% by SDS PAGE and Coomassie blue staining.
Form/Format
Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF and 0.1% 2-mercaptoethanol.
Application Notes
MS Tryptic Fingerprint: Confirmed identity as TBK1 with 50% amino acid coverage of the translated native sequence., TBK1 Kinase Assay: 3.97-50ng of this lot of enzyme phosphorylated 200uM CK1tide in the assay. Assay background was subtracted from the actual counts to yield the results.
Specific Activity
~300U/mg, where one unit of TBK1 activity is defined as 1nmol phosphate incorporated into 200uM CK1tide (KRRRAL[pS]VASLPGL) per minute at 30 degree C with a final ATP concentration of 100uM.
Preparation and Storage
For long-term storage, store at -70 degree C. Aliquots are stable for at least 6 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Related Product Information for NFkB Activating Kinase, active active protein
N-terminal His6-tagged, recombinant, full length, human TBK1, expressed by baculovirus in Sf21 cells.
The activation of NF-kappaB by receptors in the tumor necrosis factor (TNF) receptor and Toll/interleukin-1 (IL-1) receptor families requires the TRAF family of adaptor proteins. Receptor oligomerization causes the recruitment of TRAFs to the receptor complex, followed by the activation of a kinase cascade that results in the phosphorylation of IkappaB. TANK is a TRAF-binding protein that can inhibit the binding of TRAFs to receptor tails and can also inhibit NF-kappaB activation by these receptors. However, TANK also displays the ability to stimulate TRAF-mediated NF-kappaB activation. In this report, we investigate the mechanism of the stimulatory activity of TANK. We find that TANK interacts with TBK1 (TANK-binding kinase 1), a novel IKK-related kinase that can activate NF-kappaB in a kinase-dependent manner. TBK1, TANK and TRAF2 can form a ternary complex, and complex formation appears to be required for TBK1 activity. Kinase-inactive TBK1 inhibits TANK-mediated NF-kappaB activation but does not block the activation mediated by TNF-alpha, IL-1 or CD40. The TBK1-TANK-TRAF2 signaling complex functions upstream of NIK and the IKK complex and represents an alternative to the receptor signaling complex for TRAF-mediated activation of NF-kappaB.
The activation of NF-kappaB by receptors in the tumor necrosis factor (TNF) receptor and Toll/interleukin-1 (IL-1) receptor families requires the TRAF family of adaptor proteins. Receptor oligomerization causes the recruitment of TRAFs to the receptor complex, followed by the activation of a kinase cascade that results in the phosphorylation of IkappaB. TANK is a TRAF-binding protein that can inhibit the binding of TRAFs to receptor tails and can also inhibit NF-kappaB activation by these receptors. However, TANK also displays the ability to stimulate TRAF-mediated NF-kappaB activation. In this report, we investigate the mechanism of the stimulatory activity of TANK. We find that TANK interacts with TBK1 (TANK-binding kinase 1), a novel IKK-related kinase that can activate NF-kappaB in a kinase-dependent manner. TBK1, TANK and TRAF2 can form a ternary complex, and complex formation appears to be required for TBK1 activity. Kinase-inactive TBK1 inhibits TANK-mediated NF-kappaB activation but does not block the activation mediated by TNF-alpha, IL-1 or CD40. The TBK1-TANK-TRAF2 signaling complex functions upstream of NIK and the IKK complex and represents an alternative to the receptor signaling complex for TRAF-mediated activation of NF-kappaB.
Product Categories/Family for NFkB Activating Kinase, active active protein
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Product Notes
The NFkB Activating Kinase, active (Catalog #AAA636289) is an Active Protein produced from Sf21 cells and is intended for research purposes only. The product is available for immediate purchase. MS Tryptic Fingerprint: Confirmed identity as TBK1 with 50% amino acid coverage of the translated native sequence., TBK1 Kinase Assay: 3.97-50ng of this lot of enzyme phosphorylated 200uM CK1tide in the assay. Assay background was subtracted from the actual counts to yield the results. Researchers should empirically determine the suitability of the NFkB Activating Kinase, active for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFkB Activating Kinase, active, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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