Phosphoinositide 3 kinase, p85 alpha, p110 beta, active Active Protein | PIK3R2 active protein

Phosphoinositide 3 kinase, p85 alpha, p110 beta, active, Recombinant, Human (PI3K)

Cat. Num. AAA637646

• Gene Names: PIK3R2; p85; MPPH; P85B; p85-BETA
• Purity: Purified; Purified using Ni2+/NTA-agarose. ~87% by SDS-PAGE and Coomassie blue staining.
• Synonyms: Phosphoinositide 3 kinase; p85 alpha; p110 beta; active; Recombinant; Human (PI3K); PIK3R2 active protein

• Host: Sf21 insect cells
• Purity/Purification: Purified; Purified using Ni2+/NTA-agarose. ~87% by SDS-PAGE and Coomassie blue staining.
• Form/Format: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine and 0.1% 2-mercaptoethanol.

• Application Notes: Kinase Assay, MS Tryptic Fingerprint: Confirmed identity as PI3 Kinase (p110b/p85a) with 26% amino acid coverage of the translated native p110b sequence and 32% amino acid coverage of the translated native p85a.
• Specific Activity: ~40U/mg, where one unit of PI3 Kinase (p110b/p85a) activity is defined as 1nmol phosphatidylinositol 3,4,5-trisphosphate formed per minute at 22 degree C with a final ATP concentration of 10uM.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Figure: Sample enzyme activity data. See certificate of analysis for lot specific activity data.)

Related Product Information for PIK3R2 active protein

The PI3Kb isoform can be activated by insulin via the insulin receptor to initiate a cascade of events that control cell growth and metabolism. The activation of PI3Kb is mediated by the p85 regulatory subunit binding to tyrosine phosphorylated insulin receptor substrate (IRS) proteins (e.g. IRS-1 and IRS-2). It was also shown that PI3Kb is involved in apoptosis in human colon carcinoma cells. Injection of neutralizing antibodies specific to p110b in WiDr, HCT116 and CO 115 adenocarcinoma cells inhibited de novo DNA synthesis. PI3Kb is the major PI3K isoform required for apoptotic cell and Fc-g receptor mediated phagocytosis shown for primary mouse macrophages and the Jurkat human leukemia T cell line. It was shown by several research groups that the catalytic subunit of PI3Kb can be activated by Gbg subunits of G-protein coupled receptors.

Product Categories/Family for PIK3R2 active protein

Molecular Biology; MB-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4826908
• NCBI GeneID: 5296
• NCBI Accession #: NP_005018.1
• NCBI GenBank Nucleotide #: NM_005027.3
• UniProt Accession #: O00459; Q5EAT5; Q9UPH9
• Molecular Weight: 81,545 Da
• NCBI Official Full Name: phosphatidylinositol 3-kinase regulatory subunit beta
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• NCBI Official Symbol: PIK3R2
• NCBI Official Synonym Symbols: p85; MPPH; P85B; p85-BETA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PI3K regulatory subunit beta; ptdIns-3-kinase regulatory subunit p85-beta; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta)
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit beta
• Protein Family: Phosphatidylinositol 3-kinase regulatory
• UniProt Gene Name: PIK3R2
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta
• UniProt Entry Name: P85B_HUMAN

NCBI Description

Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

PIK3R2: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Kinase, lipid

Chromosomal Location of Human Ortholog: 19q13.2-q13.4

Cellular Component: cytosol; nucleus; phosphoinositide 3-kinase complex

Molecular Function: protein binding; protein heterodimerization activity; 1-phosphatidylinositol-3-kinase activity; phosphoinositide 3-kinase regulator activity; receptor tyrosine kinase binding; protein phosphatase binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide 3-kinase cascade; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; regulation of phosphoinositide 3-kinase activity; T cell receptor signaling pathway; protein transport; regulation of small GTPase mediated signal transduction; cellular response to insulin stimulus; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process; positive regulation of transcription factor import into nucleus; insulin receptor signaling pathway; innate immune response; cell glucose homeostasis; regulation of autophagy; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration

Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 1