Protein Kinase C iota, active Active Protein | pkc-3 active protein

Protein Kinase C iota, active, Recombinant, Human (PKCi)

Cat. Num. AAA635833

• Purity: Purified; ~80% by SDS-PAGE and Coomassie blue staining. Purified using Ni2+/NTA-agarose.
• Synonyms: Protein Kinase C iota; active; Recombinant; Human (PKCi); pkc-3 active protein

• Host: Sf21 cells
• Purity/Purification: Purified; ~80% by SDS-PAGE and Coomassie blue staining. Purified using Ni2+/NTA-agarose.
• Form/Format: Supplied as a liquid in 20mM HEPES/NaOH, pH 7.4, 250mM sodium chloride, 50% glycerol, 5mM DTT, 2mM EDTA, 2mM EGTA, 0.05% Triton X-100.

• Application Notes: Suitable for use in Kinase Assays.,; Dilution: Kinase Assay: 1.6-4.4ng of this lot of enzyme phosphorylated 50uM PKCtide Substrate Peptide in the assay. Assay background was subtracted from the actual counts to yield the results.
• Specific Activity: ~800U/mg, where one unit of PKC iota activity is defined as 1nmol phosphate incorporated into 50uM PKCe Substrate Peptide (ERMRPRKRQGSVRRRV) per minute at 30 degree C with a final ATP concentration of 100uM.
• Molecular Formula: 68.6kD
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Related Product Information for pkc-3 active protein

N-terminal His6-tagged, recombinant full-length human PKC iota expressed in Sf21 cells.

Product Categories/Family for pkc-3 active protein

Molecular Biology; MB-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 309371500
• UniProt Accession #: A8WUG4
• Molecular Weight: 67,842 Da
• NCBI Official Full Name: Protein CBR-PKC-3
• UniProt Protein Name: Protein kinase C-like 3
• Protein Family: Protein kinase
• UniProt Gene Name: pkc-3
• UniProt Synonym Gene Names: aPKC3
• UniProt Entry Name: KPC3_CAEBR

Uniprot Description

Function: Required for the normal progression of embryogenesis and viability of the organism. Plays an indispensable role in establishing embryonic polarity and in recruiting and maintaining par-6 to the periphery, through interaction with par-3. Required for epithelial cell polarity in the distal spermatheca. Phosphorylates serine residues of num-1

By similarity.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium

By similarity.

Subunit structure: Interaction with par-3 required for the peripheral localization of par-6 and to form a par-3/par-6/pkc-3 complex, which is activated when cdc-42 interacts with par-6. Binds avidly to the phosphotyrosine interaction domain (PID) of a novel pkc-3 adapter protein num-1, which enables tethering and targeting of pkc-3 to the cell periphery

By similarity.

Subcellular location: Cytoplasm

By similarity. Cytoplasm › cytoskeleton

By similarity. Note: Targeted and anchored at the apical surface (villi) of intestinal and pharyngeal cells, and in proximity with the cortical actin cytoskeleton that lies under the plasma membrane. Tightly bound to organelles/cytoskeleton in six of the seven developmental stages. Accumulation in cytoplasm is restricted to L1 larvae and adults. Colocalized with par-3 at the anterior cortex of the 1-cell embryo

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 OPR domain.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain.