Mouse Splicing Factor 3B Subunit 3 (SF3B3) ELISA Kit | SF3B3 elisa kit

Mouse Splicing Factor 3B Subunit 3 (SF3B3) ELISA Kit

Cat. Num. AAA2707058

• Gene Names: SF3B3; RSE1; SAP130; SF3b130; STAF130
• Reactivity: Mouse
• Synonyms: Splicing Factor 3B Subunit 3 (SF3B3); Mouse Splicing Factor 3B Subunit 3 (SF3B3) ELISA Kit; RSE1; SAP130; SF3b130; STAF130; Spliceosome-associated protein 130; Pre-mRNA-splicing factor SF3b 130 kDa subunit; SF3B3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Splicing Factor 3B Subunit 3 (SF3B3).; No significant cross-reactivity or interference between Splicing Factor 3B Subunit 3 (SF3B3) and analogues was observed.
• Sequence Length: 399

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Splicing Factor 3B Subunit 3 (SF3B3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Splicing Factor 3B Subunit 3 (SF3B3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SF3B3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Splicing Factor 3B Subunit 3 (SF3B3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Splicing Factor 3B Subunit 3 (SF3B3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Splicing Factor 3B Subunit 3 (SF3B3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Splicing Factor 3B Subunit 3 (SF3B3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 54112121
• NCBI GeneID: 23450
• NCBI Accession #: NP_036558.3
• NCBI GenBank Nucleotide #: NM_012426.4
• UniProt Accession #: Q15393; Q6NTI8; Q96GC0; Q9BPY2; Q9UFX7; Q9UJ29
• Molecular Weight: 44,606 Da
• NCBI Official Full Name: splicing factor 3B subunit 3
• NCBI Official Synonym Full Names: splicing factor 3b subunit 3
• NCBI Official Symbol: SF3B3
• NCBI Official Synonym Symbols: RSE1; SAP130; SF3b130; STAF130
• NCBI Protein Information: splicing factor 3B subunit 3
• UniProt Protein Name: Splicing factor 3B subunit 3
• Protein Family: Splicing factor
• UniProt Gene Name: SF3B3
• UniProt Synonym Gene Names: KIAA0017; SAP130; SF3b130; SAP 130

NCBI Description

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).