Rabbit Proteasome alpha 2 Polyclonal Antibody

Proteasome alpha 2 (PSMA2)

Cat. Num. AAA626294

• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: Proteasome alpha 2; Polyclonal Antibody; Proteasome alpha 2 (PSMA2); Anti -Proteasome alpha 2 (PSMA2); anti-Proteasome alpha 2 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Detects endogenous levels of total human PSMA2 protein. Species Crossreactivity: Monkey, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.

• Applicable Applications for anti-Proteasome alpha 2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Immunocytochemistry and Western Blot.
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to residues surrounding Thr223 of human PSMA2 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from various cell lines using MBS626294.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HeLa cells using MBS626294 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).)

Related Product Information for anti-Proteasome alpha 2 antibody

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with 7 distinct subunits. The two outer layers are identical rings composed of subunits (called PSMAs), and the two inner layers are identical rings composed of subunits. While the catalytic sites are located on the rings (1-3), the subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different and ten different proteasome genes have been identified in mammals (5).PA700, PA28 and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome which preferentially degrades poly-ubiquitinylated proteins (1-3). The proteasome has a broad substrate spectrum which includes cell cycle regulators, signaling molecules, tumor suppressors and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune response, protein folding and disease progression (6-9).

Product Categories/Family for anti-Proteasome alpha 2 antibody

Antibodies; Abs to Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 81157903
• NCBI Official Full Name: proteasome alpha 2