Western Blot (WB)
(Western Blot analysis of Jurkat cell lysate using MBS605241. The major band detected has a molecular weight of ~64.8kD.)
Mouse Collapsin Response Mediator Protein 4 Monoclonal Antibody | anti-Dpysl3 antibody
Collapsin Response Mediator Protein 4 (CRMP4, DPYSL3, DRP-3, DRP3, ULIP, Dihydropyrimidinase-like 3)
Gene Names
Dpysl3; Crmp4; TUC-4b
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified by Protien G affinity chromatography.
Synonyms
Collapsin Response Mediator Protein 4; Monoclonal Antibody; Collapsin Response Mediator Protein 4 (CRMP4; DPYSL3; DRP-3; DRP3; ULIP; Dihydropyrimidinase-like 3); Anti -Collapsin Response Mediator Protein 4 (CRMP4; anti-Dpysl3 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
9G340
Specificity
Recognizes human DRP-3. Species Crossreactivity: mouse and rat.
Purity/Purification
Purified by Protien G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Concentration
~1mg/ml (after reconstitution) (varies by lot)
Applicable Applications for anti-Dpysl3 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Direct ELISA and Western Blot.
Other applications have not been tested.
Optimal dilution to be determined by the researcher.
Other applications have not been tested.
Optimal dilution to be determined by the researcher.
Immunogen
Partial recombinant protein corresponding to aa457-555 of human DRP-3 with a GST tag.
Grade
Affinity Purified
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot analysis of Jurkat cell lysate using MBS605241. The major band detected has a molecular weight of ~64.8kD.)
Western Blot (WB)
(Western Blot analysis of Jurkat cell lysate using MBS605241. The major band detected has a molecular weight of ~64.8kD.)
Related Product Information for anti-Dpysl3 antibody
DRP-3 belongs to the hydantoinase/dihydropyrimidase subfamily. It is required for signalling by class 3 semaphorins and remodelling of the cytoskeleton. It also plays a role in axon guidance, neuronal growth cone collapse and cell migration. It exists as a homotetramer, and heterotetramer with CRMP1, DPYSL2, DPYSL4 or DPYSL5. The unprocessed precursor is 570 amino acids long and has an estimated molecular weight of 61.9kD. It is mainly expressed in heart and skeletal muscle, but is also strongly expressed in fetal brain and spinal cord. It localizes to the cytoplasm and growth cone. This protein interacts with synaptic vesicle protein 2 and SH3A domain of intersectin.
Product Categories/Family for anti-Dpysl3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
collapsin response mediator protein 4
NCBI Official Synonym Full Names
dihydropyrimidinase-like 3
NCBI Official Symbol
Dpysl3
NCBI Official Synonym Symbols
Crmp4; TUC-4b
NCBI Protein Information
dihydropyrimidinase-related protein 3; DRP-3; CRMP-4; TOAD-64/Ulip/CRMP protein 4b; collapsin response mediator protein 4
UniProt Protein Name
Dihydropyrimidinase-related protein 3
Protein Family
UniProt Gene Name
Dpysl3
UniProt Synonym Gene Names
Crmp4
UniProt Entry Name
DPYL3_RAT
NCBI Description
protein expressed in the nervous system during development that may be involved in neuronal plasticity, neurite growth and extension, and may serve as a ligand of the extracellular matrix during brain development [RGD]
Uniprot Description
CRMP-4: Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration. Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase
Cellular Component: filamentous actin; extracellular space; growth cone; lamellipodium; cytosol
Molecular Function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides; phosphoprotein binding; SH3 domain binding
Biological Process: actin crosslink formation; positive regulation of filopodium formation; nervous system development; actin filament bundle formation; neuron development; response to axon injury; negative regulation of cell migration; pyrimidine base catabolic process; protein homooligomerization
Research Articles on Dpysl3
Similar Products
Product Notes
The Dpysl3 dpysl3 (Catalog #AAA605241) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Collapsin Response Mediator Protein 4 (CRMP4, DPYSL3, DRP-3, DRP3, ULIP, Dihydropyrimidinase-like 3) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Collapsin Response Mediator Protein 4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Direct ELISA and Western Blot. Other applications have not been tested. Optimal dilution to be determined by the researcher. Researchers should empirically determine the suitability of the Dpysl3 dpysl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collapsin Response Mediator Protein 4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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