Rabbit anti-Human, Mouse Collagen VII alpha1 Polyclonal Antibody | anti-COL7A1 antibody

Collagen VII alpha1 Antibody

Cat. Num. AAA9609218

• Gene Names: COL7A1; EBD1; EBR1; EBDCT; NDNC8
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: Collagen VII alpha1; Polyclonal Antibody; Collagen VII alpha1 Antibody; Alpha 1 type VII collagen; CO7A1_HUMAN; Col7a1; Collagen alpha 1(VII) chain; Collagen alpha-1(VII) chain; Collagen type VII alpha 1; Collagen VII alpha 1 polypeptide; CollagenVII; EBD 1; EBD1; EBDCT; EBR 1; EBR1; LC collagen; Long chain collagen; Long-chain collagen; anti-COL7A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Collagen VII alpha1 antibody detects endogenous levels of total Collagen VII alpha1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 2944

• Applicable Applications for anti-COL7A1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human Collagen VII alpha1
• Subcellular Location: Secreted > Extracellular Space > Extracellular Matrix > Basement Membrane.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of Collagen VII alpha1 using HeLa whole cell lysates)

Related Product Information for anti-COL7A1 antibody

Function: Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
Subunit Structure: Homotrimer. Interacts with MIA3/TANGO1; facilitating its loading into transport carriers and subsequent secretion.
Post-translational Modifications: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

NCBI and Uniprot Product Information

• NCBI GI #: 4502961
• NCBI GeneID: 1294
• NCBI Accession #: NP_000085.1
• NCBI GenBank Nucleotide #: NM_000094.3
• UniProt Accession #: Q02388; Q14054; Q16507
• Molecular Weight: Observed: 295 kDa; Predicted: 296 kDa
• NCBI Official Full Name: collagen alpha-1(VII) chain
• NCBI Official Synonym Full Names: collagen type VII alpha 1 chain
• NCBI Official Symbol: COL7A1
• NCBI Official Synonym Symbols: EBD1; EBR1; EBDCT; NDNC8
• NCBI Protein Information: collagen alpha-1(VII) chain
• UniProt Protein Name: Collagen alpha-1(VII) chain
• UniProt Gene Name: COL7A1
• UniProt Synonym Gene Names: LC collagen

NCBI Description

This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq, Jul 2008]

Uniprot Description

Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.