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Western Blot (WB) (Western blot analysis of anti-anti-DGK delta Pab in Jurkat cell line lysates (35ug/lane). DGK delta (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse DGKD Polyclonal Antibody | anti-DGKD antibody

DGKD, CT (Diacylglycerol Kinase delta, Diglyceride Kinase delta, DGK-delta, DAG Kinase delta, 130kD Diacylglycerol Kinase, KIAA0145) (PE)

Gene Names
DGKD; dgkd-2; DGKdelta
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
DGKD; Polyclonal Antibody; CT (Diacylglycerol Kinase delta; Diglyceride Kinase delta; DGK-delta; DAG Kinase delta; 130kD Diacylglycerol Kinase; KIAA0145) (PE); anti-DGKD antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human DGKD. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-DGKD antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human DGKD (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-anti-DGK delta Pab in Jurkat cell line lysates (35ug/lane). DGK delta (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-anti-DGK delta Pab in Jurkat cell line lysates (35ug/lane). DGK delta (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Product Categories/Family for anti-DGKD antibody
References
1. Sakane, F., et al., J. Biol. Chem. 271(14):8394-8401 (1996). 2. Nagase, T., et al., DNA Res. 2(4):167-174 (1995).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
134,525 Da
NCBI Official Full Name
diacylglycerol kinase delta isoform 1
NCBI Official Synonym Full Names
diacylglycerol kinase, delta 130kDa
NCBI Official Symbol
DGKD
NCBI Official Synonym Symbols
dgkd-2; DGKdelta
NCBI Protein Information
diacylglycerol kinase delta; DGK-delta; DAG kinase delta; diglyceride kinase delta
UniProt Protein Name
Diacylglycerol kinase delta
Protein Family
UniProt Gene Name
DGKD
UniProt Synonym Gene Names
KIAA0145; DAG kinase delta; DGK-delta
UniProt Entry Name
DGKD_HUMAN

NCBI Description

This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

DGKD: May function as signaling molecule. Belongs to the eukaryotic diacylglycerol kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerophospholipid; Lipid Metabolism - glycerolipid; Kinase, lipid; EC 2.7.1.107; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane

Molecular Function: diacylglycerol binding; protein binding; protein homodimerization activity; protein heterodimerization activity; metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; second-messenger-mediated signaling; platelet activation; multicellular organismal development; endocytosis; signal transduction; response to organic substance; protein transport; protein kinase C activation; diacylglycerol metabolic process; blood coagulation; cell growth; phosphorylation; protein homooligomerization

Research Articles on DGKD

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Product Notes

The DGKD dgkd (Catalog #AAA6472415) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DGKD, CT (Diacylglycerol Kinase delta, Diglyceride Kinase delta, DGK-delta, DAG Kinase delta, 130kD Diacylglycerol Kinase, KIAA0145) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DGKD can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DGKD dgkd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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