Western Blot (WB)
(Western Blot detection of NFE2L1 in Hela, K562, CEM&U2OS cell lysates using NFE2L1 antibody (1:1000 diluted).)
Mouse anti-Human NFE2L1 Monoclonal Antibody | anti-NFE2L1 antibody
NFE2L1 Monoclonal Antibody
Reactivity
Human
Applications
Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
NFE2L1; Monoclonal Antibody; NFE2L1 Monoclonal Antibody; NFE2L1HBZ17; TCF11; TCF-11; Transcription factor HBZ17; Transcription factor LCR-F1; Transcription factor 11; Locus control region-factor 1; NF-E2-related factor 1; NFE2-related factor 1; Nuclear factor erythroid 2-related factor 1; anti-NFE2L1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Specificity
This antibody detects endogenous levels of NFE2L1 and does not cross-react with related proteins
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
772
Applicable Applications for anti-NFE2L1 antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Immunogen
Recombinant full length Human NFE2L1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot detection of NFE2L1 in Hela, K562, CEM&U2OS cell lysates using NFE2L1 antibody (1:1000 diluted).)
Western Blot (WB)
(Western Blot detection of NFE2L1 in Hela, K562, CEM&U2OS cell lysates using NFE2L1 antibody (1:1000 diluted).)
Related Product Information for anti-NFE2L1 antibody
The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (MAREs). A family of NF-E2-related proteins, which are collectively known as the cap n collar (CNC) family and include NFE2L1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, Jun B and Jun D) to activate gene expression, specifically the detoxifying enzyme NQO1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Band Size: 67KDa
Observed Band Size: 72KDa
Observed Band Size: 72KDa
NCBI Official Full Name
endoplasmic reticulum membrane sensor NFE2L1 isoform 1
UniProt Protein Name
Nuclear factor erythroid 2-related factor 1
Protein Family
UniProt Gene Name
NFE2L1
UniProt Synonym Gene Names
HBZ17; NRF1; TCF11; NF-E2-related factor 1; NFE2-related factor 1; TCF-11
UniProt Entry Name
NF2L1_HUMAN
Uniprot Description
NFE2L1: Activates erythroid-specific, globin gene expression. Interacts with KEAP1. Belongs to the bZIP family. CNC subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 17q21.3
Cellular Component: endoplasmic reticulum membrane; integral to membrane; nucleus
Molecular Function: transcription cofactor activity; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; anatomical structure morphogenesis; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; heme biosynthetic process
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Product Notes
The NFE2L1 nfe2l1 (Catalog #AAA3014342) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NFE2L1 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NFE2L1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the NFE2L1 nfe2l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFE2L1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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