Mouse anti-Human, Mouse Heterochromatin Protein 1, alpha Monoclonal Antibody | anti-LOC100689444 antibody
Heterochromatin Protein 1, alpha (HP1a)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
AscitesAscites
Synonyms
Heterochromatin Protein 1; alpha; Monoclonal Antibody; alpha (HP1a); Anti -Heterochromatin Protein 1; anti-LOC100689444 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
2Q2288
Specificity
Recognizes mouse Heterochromatin protein-1 alpha (HP1a) between aa67-119. Species Crossreactivity: human.
Purity/Purification
Ascites
Ascites
Ascites
Form/Format
Supplied as a liquid. No preservative added.
Applicable Applications for anti-LOC100689444 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Dilution: ELISA: 1:500-1:5000
Western Blot: 1:500-1:5000
Immunocytochemistry: 1:500-1:5000
Immunohistochemistry: 1:500-1:5000
Immunoprecipitation: 1:500-1:5000
Western Blot: 1:500-1:5000
Immunocytochemistry: 1:500-1:5000
Immunohistochemistry: 1:500-1:5000
Immunoprecipitation: 1:500-1:5000
Immunogen
Recombinant mouse HP1a.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-LOC100689444 antibody
Heterochromatin protein 1 (HP1) is a nonhistone chromosomal protein, first identified in Drosophila, that plays a dose-dependent role in gene silencing. Three orthologs, HP1alpha, HP1beta, and HP1gamma, have been characterized in mammals. While HP1alpha and HP1beta have been unambiguously localized in heterochromatin by immunocytochemical methods, HP1gamma has been found either exclusively associated with euchromatin or present in both euchromatin and heterochromatin. Here, using an antibody directed against a peptide epitope at the carboxyl-terminal end of the molecule, we localize HP1gamma in both euchromatin and heterochromatin compartments of interphase nuclei, as well as in the pericentromeric chromatin and arms of mitotic chromosomes of 3T3 cells. This dual location was also observed in nuclei expressing HP1gamma as a fusion protein with green fluorescent protein. In contrast, when the distribution of HP1gamma was analyzed with antibodies directed against an amino-terminal epitope, the protein was detectable in euchromatin and not in heterochromatin, except for transient heterochromatin staining during the late S phase, when the heterochromatin undergoes replication. These data suggest that the controversial immunolocalization of HP1gamma in chromatin is due to the use of antibodies directed against topologically distinct epitopes, those present at the amino-terminal end of the molecule being selectively masked in nonreplicative heterochromatin.
Product Categories/Family for anti-LOC100689444 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
heterochromatin protein 1 alpha
NCBI Official Symbol
LOC100689444
NCBI Protein Information
heterochromatin protein 1 alpha
Similar Products
Product Notes
The LOC100689444 (Catalog #AAA600145) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Heterochromatin Protein 1, alpha (HP1a) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Heterochromatin Protein 1, alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Dilution: ELISA: 1:500-1:5000 Western Blot: 1:500-1:5000 Immunocytochemistry: 1:500-1:5000 Immunohistochemistry: 1:500-1:5000 Immunoprecipitation: 1:500-1:5000. Researchers should empirically determine the suitability of the LOC100689444 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heterochromatin Protein 1, alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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