Rabbit anti-Human, Mouse CYP2R1 Polyclonal Antibody | anti-CYP2R1 antibody

CYP2R1, ID (Vitamin D 25-hydroxylase, Cytochrome P450 2R1) (Biotin)

Cat. Num. AAA6471604

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CYP2R1; Polyclonal Antibody; ID (Vitamin D 25-hydroxylase; Cytochrome P450 2R1) (Biotin); anti-CYP2R1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP2R1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CYP2R1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CYP2R1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CYP2R1 Antibody (Center) in NCI-H460 cell line(lane 1) and mouse liver tissue(lane 2) lysates (35ug/lane). CYP2R1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with CYP2R1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-CYP2R1 antibody

Antibodies; Enzymes; Cytochrome

References

1. Ramos-Lopez, E., Diabetes Obes Metab 10(8), 683-685 (2008). 2. Strushkevich, N., J. Mol. Biol. 380(1), 95-106 (2008). 3. Ramos-Lopez, E., Diabetes Metab. Res. Rev. 23(8), 631-636 (2007). 4. Nelson, D.R., Pharmacogenetics 14(1), 1-18 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 45267826
• NCBI GeneID: 120227
• NCBI Accession #: NP_078790
• NCBI GenBank Nucleotide #: NM_024514
• UniProt Accession #: Q6VVX0; Q2M3H3; Q5RT65
• Molecular Weight: 57,359 Da
• NCBI Official Full Name: vitamin D 25-hydroxylase
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily R, polypeptide 1
• NCBI Official Symbol: CYP2R1
• NCBI Protein Information: vitamin D 25-hydroxylase; cytochrome P450 2R1; cytochrome P450, family 2, R1
• UniProt Protein Name: Vitamin D 25-hydroxylase
• Protein Family: Vitamin D 25-hydroxylase
• UniProt Gene Name: CYP2R1
• UniProt Entry Name: CP2R1_HUMAN

Uniprot Description

CYP2R1: Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D(2) and D(3). Defects in CYP2R1 are the cause of rickets vitamin D- dependent type 1B (VDDR1B); also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. The patients sera have low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activityand low levels of 25-hydroxyvitamin D. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.13.15; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; steroid hydroxylase activity; oxygen binding; vitamin D3 25-hydroxylase activity

Biological Process: steroid metabolic process; organic acid metabolic process; vitamin metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; vitamin D metabolic process

Disease: Vitamin D Hydroxylation-deficient Rickets, Type 1b