Rabbit ANGPT2 Antibody | anti-ANGPT2 antibody

ANGPT2 Antibody

Cat. Num. AAA8501672

• Gene Names: ANGPT2; ANG2; AGPT2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: ANGPT2; ANGPT2 Antibody; ANG-2; ANG2; AGPT2; Angiopoietin-2; anti-ANGPT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 495

• Applicable Applications for anti-ANGPT2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human ANGPT2
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of HepG2 cell lysateusing ANGPT2 antibody.)

Related Product Information for anti-ANGPT2 antibody

Angiopoietins are a family of Tie receptor ligands. There are four angiopoietins discovered so far: angiopoietins 1, 2, 3 and 4 (Ang1, 2, 3, and 4) (1-3). Ang1 binds to the Tie-2 receptor and leads to its autophosphorylation and subsequent activation of downstream signaling pathways. It plays an important role in blood vessel formation, maturation and subsequent stabilization (1, 4, 5). Ang2 is an endothelium-specific growth factor that functions as an antagonist to Ang1, promotes vascular associated proinflammatory function, destabilizes quiescent endothelium, leads to vascular leakage and vascular destablization and remodeling (2, 6, 7). Ang2 is selectively expressed in many tumor tissues where, combined with other growth factors such as VEGF, it can promote vascular remodeling, angiogenesis and inflammation (7-9).

Product Categories/Family for anti-ANGPT2 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Davis, S. et al. (1996) Cell 87, 1161-9. 2. Maisonpierre, P.C. et al. (1997) Science 277, 55-60. 3. Valenzuela, D.M. et al. (1999) Proc Natl Acad Sci USA 96, 1904-9. 4. Suri, C. et al. (1996) Cell 87, 1171-80. 5. Thurston, G. et al. (1999) Science 286, 2511-4. 6. Gale, N.W. et al. (2002) Dev Cell 3, 411-23. 7. Fiedler, U. et al. (2006) Nat Med 12, 235-9. 8. Tait, C.R. and Jones, P.F. (2004) J Pathol 204, 1-10. 9. Tsutsui, S. et al. (2006) Breast Cancer Res Treat 98, 261-6.

NCBI and Uniprot Product Information

• NCBI GI #: 169646749
• NCBI GeneID: 285
• NCBI Accession #: NP_001112359.1
• NCBI GenBank Nucleotide #: NM_001118887.1
• UniProt Accession #: O15123; Q9NRR7; Q9P2Y7; A0AV38; A8K205; B7ZLM7
• Molecular Weight: 57kDa
• NCBI Official Full Name: angiopoietin-2 isoform b
• NCBI Official Synonym Full Names: angiopoietin 2
• NCBI Official Symbol: ANGPT2
• NCBI Official Synonym Symbols: ANG2; AGPT2
• NCBI Protein Information: angiopoietin-2
• UniProt Protein Name: Angiopoietin-2
• Protein Family: Angiopoietin
• UniProt Gene Name: ANGPT2
• UniProt Synonym Gene Names: ANG-2

NCBI Description

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.