Western Blot (WB)
(Western blot analysis of CBX1 Antibody (C-term) in MCF-7, A2058, Y79 cell line lysates (35ug/lane). CBX1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human Heterochromatin Protein p25 Polyclonal Antibody
Heterochromatin Protein p25, CT (p25beta, HP1Hsbeta, CBX1, M31, Modifier 1 Protein, Heterochromatin Protein 1 Homolog beta, HP1 beta, Chromobox Protein Homolog 1, CBX)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Western Blot (WB)
(Western blot analysis of CBX1 Antibody (C-term) in MCF-7, A2058, Y79 cell line lysates (35ug/lane). CBX1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CBX1 Antibody (C-term) in MCF-7, A2058, Y79 cell line lysates (35ug/lane). CBX1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(CBX1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CBX1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(CBX1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CBX1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(CBX1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CBX1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
CBX1: Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane. Homodimer. Interacts directly with CHAF1A, EMSY, LBR, TIF1/TIF1A and TRIM28/TIF1B PXVXL motif via the chromoshadow domain. Interacts directly with histone H3 methylated at 'Lys-9' via the chromo domain. Interacts with SUV39H1 and SETDB1, SUV420H1 and SUV420H2. Interacts with PRDM6. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1. Expressed in all adult and embryonic tissues.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 17q21.32
Cellular Component: nucleoplasm; centric heterochromatin; male pronucleus; spindle; female pronucleus; nuclear heterochromatin; chromatin; chromosome, pericentric region; chromocenter
Molecular Function: protein binding; enzyme binding; protein homodimerization activity; chromatin binding
Biological Process: negative regulation of transcription, DNA-dependent
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Product Notes
The Heterochromatin Protein p25 (Catalog #AAA642736) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Heterochromatin Protein p25, CT (p25beta, HP1Hsbeta, CBX1, M31, Modifier 1 Protein, Heterochromatin Protein 1 Homolog beta, HP1 beta, Chromobox Protein Homolog 1, CBX) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Heterochromatin Protein p25 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the Heterochromatin Protein p25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heterochromatin Protein p25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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