Rabbit anti-E Coli DnaJ Polyclonal Antibody | anti-DnaJ antibody

DnaJ (E Coli) polyclonal antibody

Cat. Num. AAA565328

• Gene Names: dnaJ; ECK0015; faa; groP; grpC; JW0014
• Reactivity: E Coli
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A affinity purified.
• Synonyms: DnaJ; Polyclonal Antibody; DnaJ (E Coli) polyclonal antibody; HSP40; Heat shock protein 40; Heat shock protein J; anti-DnaJ antibody

• Host: Rabbit
• Reactivity: E Coli
• Clonality: Polyclonal
• Specificity: Recognizes bacteria DnaJ. Detects a band of ~41kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 376

• Applicable Applications for anti-DnaJ antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Immunoprecipitation (1:200); Western Blot (1:1,000, ECL); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~41kDa by Western blot.
• Immunogen: E Coli DnaJ.
• Host Note: Purified from rabbit serum.
• Preparation and Storage: Long term storage: -20°C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis: Lane 1: DnaJ Recombinant E Coli Protein.)

Related Product Information for anti-DnaJ antibody

DnaJ is a basic 41 kDa E Coli Heat shock protein which belongs to the molecular chaperone class of proteins. Bacterial DnaJ protein acts synergistically with the bacterial chaperone DnaK (Hsp70 homologue) and the other co-chaperone GrpE in suppressing eukaryotic and prokaryotic polypeptide aggregation, facilitating protein translocation through intracellular compartments or protein secretion, reactivating some partially aggregated enzymes, and activating pre-priming complex during initiation of DNA replication.

Product Categories/Family for anti-DnaJ antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 118719
• NCBI GeneID: 944753
• UniProt Accession #: P08622
• Molecular Weight: 41,100 Da
• NCBI Official Full Name: Chaperone protein DnaJ
• NCBI Official Symbol: dnaJ
• NCBI Official Synonym Symbols: ECK0015; faa; groP; grpC; JW0014
• NCBI Protein Information: chaperone Hsp40, DnaK co-chaperone
• UniProt Protein Name: Chaperone protein DnaJ
• Protein Family: DnaJ
• UniProt Gene Name: dnaJ
• UniProt Synonym Gene Names: groP
• UniProt Entry Name: DNAJ_ECOLI

NCBI Description

DnaK and DnaJ both bind to the same target peptide, forming a ternary complex. [More information is available at EcoGene: EG10240]. The DnaK system of Escherichia coli is a homolog of the eukaryotic Hsp70 chaperone system. [More information is available at EcoCyc: EG10240].

Uniprot Description

Interacts with DnaK and GrpE to disassemble a protein complex at the origins of replication of phage lambda and several plasmids. Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.