Typical Testing Data/Standard Curve (for reference only)
Rat Glutamate Receptor, Ionotropic, Delta 2 ELISA Kit | GRID2 elisa kit
Rat Glutamate Receptor, Ionotropic, Delta 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GRID2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRID2 antibody and an GRID2-HRP conjugate. The assay sample and buffer are incubated together with GRID2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRID2 concentration since GRID2 from samples and GRID2-HRP conjugate compete for the anti-GRID2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GRID2 from the sample, fewer sites are left to bind GRID2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRID2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans. [provided by RefSeq, Apr 2014]
Uniprot Description
GluR-delta2: Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID2 subfamily.
Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Channel, calcium; Membrane protein, integral
Chromosomal Location of Human Ortholog: 4q22
Cellular Component: postsynaptic membrane; integral to plasma membrane; dendrite; dendritic spine; plasma membrane; ionotropic glutamate receptor complex; synapse; cell junction
Molecular Function: extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; glutamate receptor activity; PDZ domain binding
Biological Process: heterophilic cell adhesion; synaptic transmission, glutamatergic; prepulse inhibition; transport; glutamate signaling pathway; ionotropic glutamate receptor signaling pathway; regulation of neuron apoptosis; regulation of excitatory postsynaptic membrane potential; cerebellar granule cell differentiation
Disease: Spinocerebellar Ataxia, Autosomal Recessive 18
Research Articles on GRID2
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Product Notes
The Rat GRID2 grid2 (Catalog #AAA755130) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA755130 ELISA Kit recognizes Rat GRID2. It is sometimes possible for the material contained within the vial of "Glutamate Receptor, Ionotropic, Delta 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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