Rabbit HSC70/HSP73 Polyclonal Antibody | anti-Heat shock protein 70; Hsc70; Hsp73 antibody

HSC70/HSP73 polyclonal antibody

Cat. Num. AAA565313

• Gene Names: HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Protein A affinity purified.
• Synonyms: HSC70/HSP73; Polyclonal Antibody; HSC70/HSP73 polyclonal antibody; anti-Heat shock protein 70; Hsc70; Hsp73 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human, mouse and rat HSC70 (HSP73). Detects a band of ~73kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 646

• Applicable Applications for anti-Heat shock protein 70; Hsc70; Hsp73 antibody: Western Blot (WB)
• Application Notes: Western Blot (1:1,000, colorimetric); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~73kDa by Western blot.
• Immunogen: Synthetic peptide corresponding to the sequence near the C-terminus of human HSC70 (HSP73). The sequence is completely conserved in rat, mouse, bovine, dog, chicken, monkey, and frog.
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of HSC70 (HSP73) pAb: Lane 1: HeLa Cell Lysate, Lane 2: HeLa Cell Lysate (Heat Shocked), Lane 3: PC -12 Cell Lysate, Lane 4: PC -12 Cell Lysate (Heat Shocked), Lane 5: 3T3 Cell Lysate, Lane 6: 3T3 Cell Lysate (Heat Shocked), Lane 7: HSC70 (HSP73) Recombinant Protein .)

Related Product Information for anti-Heat shock protein 70; Hsc70; Hsp73 antibody

The 70 kDa heat shock protein Hsp70 belongs to the Hsp70 family of highly-related protein isoforms ranging in size from 66 kDa to 78 kDa. Hsc70 shares close biochemical and biological ties to Hsp70, and also belongs to the Hsp70 family. These proteins include cognate members found within major intracellular compartments and highly inducible isoforms predominantly cytoplasmic or nuclear in distribution. Members of the Hsp70 family function as molecular chaperones involved in such cellular functions as protein folding, transport, maturation and degradation, operating in an ATP-dependent manner. The molecular chaperones of the Hsp70 family recognize and bind to nascent polypeptide chains or partially folded intermediates of proteins, preventing their aggregation and misfolding, and the binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. Data demonstrates that with a ubiquitin-like domain at its amino terminus and its association with the 26S proteosome in HeLa cells, Bag-1 modulates the chaperone activity of Hsc70 and Hsp70. These findings reveal Bag-1's role as a physical link between the Hsc70/Hsp70 chaperone system and the proteasome. Experimental data also shows that the ATPase domain and the substrate binding domain of Hsd70 cooperate to form a co-chaperone-chaperone complex with the synaptic vesicle cysteine string protein (csp), essential for normal neurotransmitter release.

Product Categories/Family for anti-Heat shock protein 70; Hsc70; Hsp73 antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 123648
• NCBI GeneID: 3312
• UniProt Accession #: P11142; Q9H3R6
• Molecular Weight: 53,518 Da
• NCBI Official Full Name: Heat shock cognate 71 kDa protein
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 8
• NCBI Official Symbol: HSPA8
• NCBI Official Synonym Symbols: LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
• NCBI Protein Information: heat shock cognate 71 kDa protein
• UniProt Protein Name: Heat shock cognate 71 kDa protein
• UniProt Gene Name: HSPA8
• UniProt Synonym Gene Names: HSC70; HSP73; HSPA10; LAP-1; LPS-associated protein 1
• UniProt Entry Name: HSP7C_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Heat shock protein; Chaperone; Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: cytosol; extracellular space; focal adhesion; intracellular; lysosomal lumen; lysosomal membrane; melanosome; membrane; myelin sheath; nucleolus; nucleoplasm; nucleus; plasma membrane; ribonucleoprotein complex; spliceosome; ubiquitin ligase complex

Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; protein binding; ubiquitin protein ligase binding; unfolded protein binding

Biological Process: ATP metabolic process; axon guidance; cellular response to starvation; chaperone cofactor-dependent protein folding; gene expression; negative regulation of transcription, DNA-dependent; neurotransmitter secretion; nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; post-Golgi vesicle-mediated transport; protein folding; protein refolding; protein targeting to lysosome; regulation of cell cycle; regulation of mRNA stability; regulation of protein complex assembly; regulation of protein stability; response to unfolded protein; RNA splicing; synaptic transmission; transcription, DNA-dependent; viral reproduction