Rabbit HSP40/Hdj2 Polyclonal Antibody | anti-HSP40/Hdj2 antibody

HSP40/Hdj2 polyclonal antibody

Cat. Num. AAA565733

• Gene Names: DNAJA1; DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSJ-2; HSPF4; NEDD7; hDJ-2
• Reactivity: Human, Mouse, Rat, Hamster
• Applications: Western Blot
• Purity: Protein A-affinity purified.
• Synonyms: HSP40/Hdj2; Polyclonal Antibody; HSP40/Hdj2 polyclonal antibody; Heat shock protein 40; pAb; anti-HSP40/Hdj2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Hamster
• Clonality: Polyclonal
• Specificity: Recognizes human, mouse, rat and hamster HSP40 (Hdj2). Detects a band of ~45kDa by Western blot.
• Purity/Purification: Protein A-affinity purified.
• Form/Format: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 397

• Applicable Applications for anti-HSP40/Hdj2 antibody: Western Blot (WB)
• Application Notes: Western Blot (1:1'000, colorimetric).; Detects a band of ~45kDa by Western blot.
• Immunogen: Recombinant human HSP40/Hdj2.
• Preparation and Storage: Long Term: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western Blot Analysis of HSP40/Hdj2, pAb: Lane 1: MWM, Lane 2: Hsp40/Hdj2 Recombinant Human Protein, Lane 3: HeLa Cell Lysate, Lane 4: HeLa Cell Lysate, Heat Shocked, Lane 5: PC-12 Cell Lysate, Lane 6: PC-12 Cell Lysate, Heat Shocked, Lane 7: 3T3 Cell Lysate, Lane 8: 3T3 Cell Lysate, Heat Shocked, Lane 9: CHO-K1 Cell Lysate, Lane 10: CHO-K1 Cell Lysate, Heat Shocked.)

Related Product Information for anti-HSP40/Hdj2 antibody

Hdj2 is an approximately 40kDa member of the heat shock protein (Hsp40) family, a eukaryotic homolog of the E Coli DnaJ protein. Eukaryotic DnaJ-like proteins share structural domain conservation with DnaJ namely: 1) an N-terminal ~70 amino acid J domain, 2) a glycine- and phenylalanine-rich domain, 3) a cysteine-rich zinc finger domain, and 4) a poorly conserved C-terminal domain. The human constitutive Hdj2 protein shares full domain conservation with DnaJ, as opposed to the human inducible Hdj1 which lacks the zinc-finger domain. The Hsp40 J domain regulates its interaction with Hsp70 (E Coli DnaK), increasing Hsp70 ATPase activity and in turn enhancing substrate binding by the Hsp70 chaperone. Hsp40 co-chaperones are involved in nearly all aspects of protein synthesis and secretion because of their importance in Hsp70 function, and they are also thought to have an intrinsic ability to bind and fold some misfolded proteins. Hdj2 has been specifically shown to interact with Hsc70, the constitutive form of Hsp70, in the biogenesis of the cystic fibrosis transmembrane conductance regulator (CFTR), mutations of which are known to cause inherited cystic fibrosis.

Product Categories/Family for anti-HSP40/Hdj2 antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 1706474
• NCBI GeneID: 3301
• UniProt Accession #: P31689; Q5T7Q0; Q86TL9
• Molecular Weight: 37,045 Da
• NCBI Official Full Name: DnaJ homolog subfamily A member 1
• NCBI Official Synonym Full Names: DnaJ heat shock protein family (Hsp40) member A1
• NCBI Official Symbol: DNAJA1
• NCBI Official Synonym Symbols: DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSJ-2; HSPF4; NEDD7; hDJ-2
• NCBI Protein Information: dnaJ homolog subfamily A member 1
• UniProt Protein Name: DnaJ homolog subfamily A member 1
• UniProt Gene Name: DNAJA1
• UniProt Synonym Gene Names: DNAJ2; HDJ2; HSJ2; HSPF4; HSJ-2; hDj-2

NCBI Description

This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes. [provided by RefSeq, Sep 2015]

Uniprot Description

DNAJA1: Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.

Protein type: Chaperone; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 9p21.1

Cellular Component: cytosol; membrane; ubiquitin ligase complex

Molecular Function: chaperone binding; G-protein-coupled receptor binding; Hsp70 protein binding; low-density lipoprotein receptor binding; protein binding; Tat protein binding; ubiquitin protein ligase binding

Biological Process: negative regulation of apoptosis; negative regulation of JNK activity; negative regulation of protein ubiquitination; positive regulation of apoptosis; regulation of protein transport; response to unfolded protein