Rabbit anti-E Coli GrpE Polyclonal Antibody | anti-HSP70 cofactor; HSP24 antibody

GrpE (E Coli) polyclonal antibody

Cat. Num. AAA565222

• Gene Names: grpE; ECK2610; JW2594
• Reactivity: E Coli
• Applications: Western Blot
• Purity: Protein A affinity purified.
• Synonyms: GrpE; Polyclonal Antibody; GrpE (E Coli) polyclonal antibody; anti-HSP70 cofactor; HSP24 antibody

• Host: Rabbit
• Reactivity: E Coli
• Clonality: Polyclonal
• Specificity: Recognizes bacteria GrpE. Detects a band of ~24kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 197

• Applicable Applications for anti-HSP70 cofactor; HSP24 antibody: Western Blot (WB)
• Application Notes: Western Blot (1:1,000, colorimetric); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~24kDa by Western blot.
• Immunogen: Purified E Coli GrpE.
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of GrpE: Lane 1: MW marker, Lane 2: GrpE Recombinant E Coli Protein.)

Related Product Information for anti-HSP70 cofactor; HSP24 antibody

GrpE (Glucose Regulated Protein E Coli) is encoded by a monocistronic E Coli gene, which is under the control of both s32 and s70 transcription factors. The GrpE protein is a so-called co-chaperone because it is known to assist the DnaK (Hsp70) protein to effectively carry out DnaK-dependent chaperone activity (i.e. protein folding, protein transport, disaggregation of heat inactivated proteins, activation of mutant protein).

Product Categories/Family for anti-HSP70 cofactor; HSP24 antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 121638
• NCBI GeneID: 947097
• UniProt Accession #: P09372
• Molecular Weight: 21,798 Da
• NCBI Official Full Name: Protein GrpE
• NCBI Official Symbol: grpE
• NCBI Official Synonym Symbols: ECK2610; JW2594
• NCBI Protein Information: heat shock protein
• UniProt Protein Name: Protein GrpE
• Protein Family: GrpE protein
• UniProt Gene Name: grpE
• UniProt Entry Name: GRPE_ECOLI

NCBI Description

HT_Cmplx22_Cyt: [More information is available at EcoGene: EG10416]. Review: [More information is available at EcoCyc: EG10416].

Uniprot Description

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.