Western Blot (WB)
(Western Blot Analysis of Hsp70 (fish), pAb : Lane 1: HSP70/HSP72 (Chinook Salmon) (recombinant), Lane 2: Dnak (E.Coli) (recombinant), Lane 3: RTG-2 Cell Lysate, Lane 4: HeLa Cell Lysate, Lane 5: L-929 Cell Lysate, Lane 6: Rat-2 Cell Lysate, Lane 7: MDBK Cell Lysate, Lane 8: MDCK Cell Lysate, Lane 9: Drosophila Cell Lysate, Lane 10: Chicken Heart Lysate)
Rabbit HSP70 Polyclonal Antibody | anti-HSP70 antibody
HSP70 (fish) polyclonal antibody
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Drosophila, Fish
Applications
Western Blot
Purity
Antiserum.
Synonyms
HSP70; Polyclonal Antibody; HSP70 (fish) polyclonal antibody; Hsp70; HspA1A; Heat shock protein 70; HspA1B; Hsp72; anti-HSP70 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Drosophila, Fish
Clonality
Polyclonal
Specificity
Recognizes human, mouse, rat, bovine, dog, chicken, Drosophila and fish HSP70. Detects a band of ~70kDa by Western blot.
Purity/Purification
Antiserum.
Form/Format
Liquid. Whole rabbit serum.
Sequence Length
644
Applicable Applications for anti-HSP70 antibody
Western Blot (WB)
Application Notes
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~70kDa by Western blot.
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~70kDa by Western blot.
Immunogen
Recombinant salmon Hsp70.
Preparation and Storage
Long term storage: -20 degree C
Avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
Western Blot (WB)
(Western Blot Analysis of Hsp70 (fish), pAb : Lane 1: HSP70/HSP72 (Chinook Salmon) (recombinant), Lane 2: Dnak (E.Coli) (recombinant), Lane 3: RTG-2 Cell Lysate, Lane 4: HeLa Cell Lysate, Lane 5: L-929 Cell Lysate, Lane 6: Rat-2 Cell Lysate, Lane 7: MDBK Cell Lysate, Lane 8: MDCK Cell Lysate, Lane 9: Drosophila Cell Lysate, Lane 10: Chicken Heart Lysate)
Western Blot (WB)
(Western Blot Analysis of Hsp70 (fish), pAb : Lane 1: HSP70/HSP72 (Chinook Salmon) (recombinant), Lane 2: Dnak (E.Coli) (recombinant), Lane 3: RTG-2 Cell Lysate, Lane 4: HeLa Cell Lysate, Lane 5: L-929 Cell Lysate, Lane 6: Rat-2 Cell Lysate, Lane 7: MDBK Cell Lysate, Lane 8: MDCK Cell Lysate, Lane 9: Drosophila Cell Lysate, Lane 10: Chicken Heart Lysate)
Related Product Information for anti-HSP70 antibody
The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
Product Categories/Family for anti-HSP70 antibody
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
70,976 Da
NCBI Official Full Name
Heat shock 70 kDa protein
UniProt Protein Name
Heat shock 70 kDa protein
Protein Family
UniProt Gene Name
hsp70
UniProt Synonym Gene Names
HSP70
UniProt Entry Name
HSP70_ONCTS
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Product Notes
The HSP70 hsp70 (Catalog #AAA565343) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSP70 (fish) polyclonal antibody reacts with Human, Mouse, Rat, Bovine, Chicken, Dog, Drosophila, Fish and may cross-react with other species as described in the data sheet. AAA Biotech's HSP70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Detects a band of ~70kDa by Western blot. Researchers should empirically determine the suitability of the HSP70 hsp70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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