Mouse HSP71 Monoclonal Antibody | anti-Heat shock protein 71 antibody

HSP71 (mycobacterial) monoclonal antibody (5A8)

Cat. Num. AAA565080

• Gene Names: HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
• Reactivity: Mycobacterium tuberculosis
• Applications: Western Blot
• Purity: Protein G affinity purified.
• Synonyms: HSP71; Monoclonal Antibody; HSP71 (mycobacterial) monoclonal antibody (5A8); anti-Heat shock protein 71 antibody

• Host: Mouse
• Reactivity: Mycobacterium tuberculosis
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5A8
• Specificity: Recognizes bacteria HSP71. Detects a band of ~71kDa by Western blot.
• Purity/Purification: Protein G affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 493

• Applicable Applications for anti-Heat shock protein 71 antibody: Western Blot (WB)
• Application Notes: Western Blot (1ug/ml, colorimetric); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~71kDa by Western blot.
• Immunogen: Recombinant mycobacterial Hsp71.
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of HSP71 protein, probed with HSP71 (mycobacterial) mAb (5A8).)

Related Product Information for anti-Heat shock protein 71 antibody

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Product Categories/Family for anti-Heat shock protein 71 antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 24234686
• NCBI GeneID: 3312
• NCBI Accession #: NP_694881.1
• NCBI GenBank Nucleotide #: NM_153201.3
• Molecular Weight: 53,518 Da
• NCBI Official Full Name: heat shock cognate 71 kDa protein isoform 2
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 8
• NCBI Official Symbol: HSPA8
• NCBI Official Synonym Symbols: LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
• NCBI Protein Information: heat shock cognate 71 kDa protein
• UniProt Protein Name: Heat shock cognate 71 kDa protein
• UniProt Gene Name: HSPA8
• UniProt Synonym Gene Names: HSC70; HSP73; HSPA10; LAP-1; LPS-associated protein 1
• UniProt Entry Name: HSP7C_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Heat shock protein; Chaperone; RNA-binding

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: cytosol; extracellular space; focal adhesion; intracellular; lysosomal lumen; lysosomal membrane; melanosome; membrane; myelin sheath; nucleolus; nucleoplasm; nucleus; plasma membrane; ribonucleoprotein complex; spliceosome; ubiquitin ligase complex

Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; protein binding; ubiquitin protein ligase binding; unfolded protein binding

Biological Process: ATP metabolic process; axon guidance; cellular response to starvation; chaperone cofactor-dependent protein folding; gene expression; negative regulation of transcription, DNA-dependent; neurotransmitter secretion; nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; post-Golgi vesicle-mediated transport; protein folding; protein refolding; protein targeting to lysosome; regulation of cell cycle; regulation of mRNA stability; regulation of protein complex assembly; regulation of protein stability; response to unfolded protein; RNA splicing; synaptic transmission; transcription, DNA-dependent; viral reproduction