Rabbit anti-Human, Mouse HSP70B' Polyclonal Antibody | anti-Hsp70B prime; Heat shock protein 70 B' antibody

HSP70B' polyclonal antibody

Cat. Num. AAA565172

• Gene Names: HSPA6; HSP70B'
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Protein A affinity purified.
• Synonyms: HSP70B'; Polyclonal Antibody; HSP70B' polyclonal antibody; anti-Hsp70B prime; Heat shock protein 70 B' antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Recognizes human and mouse HSP70B'. Detects a band of ~70kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 643

• Applicable Applications for anti-Hsp70B prime; Heat shock protein 70 B' antibody: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Immunoprecipitation (1:100); Western Blot (1ug/ml); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~70kDa by Western blot.
• Immunogen: Synthetic peptide corresponding to the sequence near the C-terminus of human Hsp70B'.
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of HSP70B' probed with HSP70B"pAb.)

Related Product Information for anti-Hsp70B prime; Heat shock protein 70 B' antibody

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Product Categories/Family for anti-Hsp70B prime; Heat shock protein 70 B' antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 34978357
• NCBI GeneID: 3310
• UniProt Accession #: P17066; Q1HBA8; Q8IYK7; Q9BT95
• Molecular Weight: 71,028 Da
• NCBI Official Full Name: Heat shock 70 kDa protein 6
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 6
• NCBI Official Symbol: HSPA6
• NCBI Official Synonym Symbols: HSP70B'
• NCBI Protein Information: heat shock 70 kDa protein 6
• UniProt Protein Name: Heat shock 70 kDa protein 6
• UniProt Gene Name: HSPA6
• UniProt Synonym Gene Names: HSP70B'
• UniProt Entry Name: HSP76_HUMAN

Uniprot Description

HSPA6: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Belongs to the heat shock protein 70 family.

Protein type: Heat shock protein

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: centriole; cytoplasm; cytosol; signalosome

Molecular Function: ATP binding; ATPase activity, coupled; enzyme binding; heat shock protein binding; protein binding; unfolded protein binding

Biological Process: protein refolding; response to unfolded protein