VP1 recombinant protein

VP1 recombinant

Cat. Num. AAA505050

• Purity: 0.95
• Synonyms: VP1; VP1 recombinant; RECOMBINANT ANTIGEN VP1 from human coxsackievirus B1; capsid protein; VP1 recombinant protein

• Host: E Coli
• Purity/Purification: 0.95
• Form/Format: Dry powder (lyophilized)
• Concentration: 0.67 mg/ml (varies by lot)
• Sequence Length: 2182

• Recombinant Antigen: human coxsackievirus B1 VP1 antigen (Ray et al., 1979).
• Organism: coxsaskievirus B1 (Tucson) CVB1(T)
• Tag: HIS
• Molecular Weight Information: Determined by SDS-PAGE, the protein band is between molecular markers of 25,000-35,000 Da, while relative molecular mass calculated from amino acid sequence is 26,665.6 Da.
• Observation Notes: In some cases, purified proteins run at a molecular weight which is slightly different to the theoretically calculated molecular weight maybe due to the his-tag which can produce a delay in SDS-PAGE. Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
• Buffer: 20 mM phosphate buffer pH 8, 10 mM NaCl, 0.1% polyoxyethylene (10) tridecyl ether, 250 mM imidazol and 6 M urea
• Batch Composition: Recombinant antigen with a his-tag in its N-terminus
• Storage Buffer: 20 mM phosphate buffer pH 8, 10 mM NaCl, 0.1% polyoxyethylene (10) tridecyl ether, 250 mM imidazol and 6 M urea
• Reconstitution: For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
• Preparation and Storage: Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.

Testing Data

Related Product Information for VP1 recombinant protein

the polyprotein from coxsackievirus B1 (Tucson) (CVB1) has been prepared as a recombinant antigen fused to a his-tag. It is produced from the C-terminal aa (487-646) of the VP1 protein.

Product Categories/Family for VP1 recombinant protein

Viruses

References

Ray, C. G., L. L. Minnich, and P. C. Johnson. Selective polymyositis induced by coxsackievirus B1 in mice. 1979, J. Infect. Dis. 140:239-243.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.

NCBI and Uniprot Product Information

• NCBI GI #: 31247844
• NCBI Official Full Name: polyprotein
• Protein Family: Capsid protein