Rabbit anti-Arabidopsis Thr449 S6 kinase 1 Polyclonal Antibody | anti-S6K1 antibody

Anti-Phospho-Thr449 S6 kinase 1 (S6K1)

Cat. Num. AAA502218

• Gene Names: PK1; ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 1; ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 6; ATPK1; ATPK6; ATS6K1; P70 RIBOSOMAL S6 KINASE; PK1; PK6; PROTEIN-SERINE KINASE; protein-serine kinase 1; ROTEIN-SERINE KINASE 6; S6K1
• Reactivity: Arabidopsis
• Applications: Western Blot
• Purity: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Synonyms: Thr449 S6 kinase 1; Polyclonal Antibody; Anti-Phospho-Thr449 S6 kinase 1 (S6K1); S6 Kinase 1 (Thr449); anti-S6K1 antibody

• Host: Rabbit
• Reactivity: Arabidopsis
• Clonality: Polyclonal
• Specificity: Specific for the ~53k S6K1 protein phosphorylated at Thr449. The immunolabeling of the S6K1 band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling.
• Purity/Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. (Adequate amount of material to conduct 10 mini Western blots)
• Sequence Length: 465

• Applicable Applications for anti-S6K1 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Immunogen Species: Arabidopsis
• Immunogen Info: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr449 conjugated to KLH
• Biological Significance: Ribosomal s6 kinase is a member of a family of protein kinases involved in signal transduction. The subfamily S6K has two known homologues: S6K1 and S6K2. First characterized in mammals, S6K1 is controlled by target-of-rapamycin (TOR) kinase, which plays a central regulatory role in growth signaling pathways (Dufner and Thomas 1999). Osmotic stress inhibition of S6K is mediated by the TOR kinase pathway (Mahfouz et al., 2006). The activation of mammalian S6K1 involves phosphorylation at thr389 (Pearson et al., 2005), however its orthologue in Arabidopsis suggests that plant S6K1 thr449 is its functional equivalent (Schepetilnikov et al., 2011). The phytohormone auxin triggers TOR activation, which is followed by S6K1 phosphorylation at thr449, which in turn is critical for translation reinitiation (Schepetilnikov et al., 2013). Rapamycin effectively inactivates S6K1 thr449 phosphorylation in Arabidopsis seedlings, which suppresses TOR PK activity and ultimately plant growth (Xiong Y and Sheen J, 2011).
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr449 of Arabidopsis S6K1.
• Preparation and Storage: For long term storage -20°C is recommended. Stable at -20°C for at least 1 year.

Western Blot (WB)

(Western blot of Arabidopsis lysate showing specific immunolabeling of the ~53k S6K1 phosphorylated at Thr449. Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (P-peptide), but not by the corresponding nonphospho-peptide (NP-peptide).)

Related Product Information for anti-S6K1 antibody

Affinity purified rabbit polyclonal antibody

NCBI and Uniprot Product Information

• NCBI GI #: 15231960
• NCBI GeneID: 820020
• NCBI Accession #: NP_187485.1
• NCBI GenBank Nucleotide #: NM_111707.3
• UniProt Accession #: P42818; Q8LFC1; A54141
• Molecular Weight: ~53 kDa
• NCBI Official Full Name: protein-serine kinase 1
• NCBI Official Symbol: PK1
• NCBI Official Synonym Symbols: ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 1; ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 6; ATPK1; ATPK6; ATS6K1; P70 RIBOSOMAL S6 KINASE; PK1; PK6; PROTEIN-SERINE KINASE; protein-serine kinase 1; ROTEIN-SERINE KINASE 6; S6K1
• NCBI Protein Information: protein-serine kinase 1
• UniProt Protein Name: Serine/threonine-protein kinase AtPK1/AtPK6
• UniProt Gene Name: ATPK1
• UniProt Synonym Gene Names: ATPK6; S6K1
• UniProt Entry Name: KPK1_ARATH

NCBI Description

Encodes a protein-serine kinase that phosphorylates ribosomal protein in vitro. Activation of AtS6k is regulated by 1-naphthylacetic acid and kinetin, at least in part, via a lipid kinase-dependent pathway. Involved in translational up-regulation of ribosomal proteins. Phosphorylated by PDK1. Interacts with RAPTOR1, which in turn interacts with TOR. SPK6 activity is affected by osmotic stress, and plants overexpressing S6k1 are hypersensitive to osmotic stress. The gene is expressed in all tissues examined, with highest expression level detected in metabolically active tissues.

Uniprot Description

Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.