Rabbit anti-Human Che-1 (AATF) (Ser477) Polyclonal Antibody | anti-AATF antibody

Anti-Phospho Ser477 Che-1 (AATF)

Cat. Num. AAA502195

• Gene Names: AATF; DED; BFR2; CHE1; CHE-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Che-1 (AATF) (Ser477); Polyclonal Antibody; Anti-Phospho Ser477 Che-1 (AATF); anti-AATF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Specific for the ~66k Che-1 protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to generate the antibody but not by the corresponding dephospho-peptide.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 560

• Applicable Applications for anti-AATF antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Synthetic phospho-peptide surrounding the phospho Ser477 of human Che-1.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 conjugated to KLH
• Immunogen Species: Human
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, mouse, non-human primate, rat, sheep and Xenopus
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with human tissue. It is also expected that the antibody will react with bovine, canine, mouse, non-human primates, rat, sheep and Xenopus as these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-AATF antibody

Affinity purified rabbit polyclonal antibody

References

• Burgdorf S, Leister P, Scheidtmann KH. (2004) TSG101 interacts with apoptosis-antagonizing transcription factor and enhances androgen receptor-mediated transcription by promoting its monoubiquitination. J Biol Chem. 279(17):17524-34
• Xie J, Guo Q (2004) AATF protects neural cells against oxidative damage induced by amyloid beta-peptide. Neurobiol Dis. 16(1):150-7
• Bruno T, De Nicola F, Iezzi S, Lecis D, D'Angelo C, Di Padova M, Corbi N, Dimiziani L, Zannini L, Jekimovs C, Scarsella M, Porrello A, Chersi A, Crescenzi M, Leonetti C, Khanna KK, Soddu S, Floridi A, Passananti C, Delia D, Fanciulli M. (2006) Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint. Cancer Cell. 10(6):473-86

NCBI and Uniprot Product Information

• NCBI GI #: 7657013
• NCBI GeneID: 26574
• NCBI Accession #: NP_036270.1
• NCBI GenBank Nucleotide #: NM_012138.3
• UniProt Accession #: Q9NY61; Q9P0A4; Q9UNX5; A6NCJ6; B3KQ26
• Molecular Weight: 66
• NCBI Official Full Name: protein AATF
• NCBI Official Synonym Full Names: apoptosis antagonizing transcription factor
• NCBI Official Symbol: AATF
• NCBI Official Synonym Symbols: DED; BFR2; CHE1; CHE-1
• NCBI Protein Information: protein AATF; rb-binding protein Che-1; apoptosis-antagonizing transcription factor
• UniProt Protein Name: Protein AATF
• Protein Family: Protein
• UniProt Gene Name: AATF
• UniProt Synonym Gene Names: CHE1; DED
• UniProt Entry Name: AATF_HUMAN

NCBI Description

The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. Ref.8 Ref.10 Ref.12 Ref.13

Subunit structure: Binds PAWR, POLR2J, RB1/RB, RBL1/P107, RBL2/P130, and SP1. May also bind MAPT.

Subcellular location: Nucleus › nucleolus Ref.9.

Tissue specificity: Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus. Ref.1 Ref.2

Post-translational modification: Hyperphosphorylated during the G1/S phase transition. Ref.8

Sequence similarities: Belongs to the AATF family.

Sequence caution: The sequence AAD52016.1 differs from that shown. Reason: Frameshift at positions 355, 365, 487, 498 and 503.