Rabbit anti-Human GJA3 / CX46 / Connexin 46 Polyclonal Antibody | anti-GJA3 antibody

Rabbit Polyclonal to Human GJA3 / CX46 / Connexin 46

Cat. Num. AAA244870

• Gene Names: GJA3; CX46; CZP3; CTRCT14
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Ammonium sulfate precipitation
• Synonyms: GJA3 / CX46 / Connexin 46; Polyclonal Antibody; Rabbit Polyclonal to Human GJA3 / CX46 / Connexin 46; Anti-GJA3 / CX46 / Connexin 46 Antibody (N-Terminus) IHC-plus; GJA3; Connexin-46; CX46; CZP3; Gap junction alpha-3 protein; Gap junction protein alpha 3; CAE3; Connexin 46; Human GJA3; anti-GJA3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Ammonium sulfate precipitation
• Form/Format: PBS, 0.09% sodium azide
• Concentration: 0.25 mg/ml (varies by lot)
• Sequence Length: 435

• Applicable Applications for anti-GJA3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:1000)
• Target Species: Human
• Immunogen Description: KLH conjugated synthetic peptide selected from the N-term region of human GJA3.
• Immunogen Type: Synthetic peptide
• Immunogen: GJA3 / CX46 / Connexin 46 antibody was raised against kLH conjugated synthetic peptide selected from the N-term region of human GJA3.
• Antigen Modification: N-Terminus
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-GJA3 / Connexin 46 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot of GJA3 Antibody (N-term) in Jurkat cell line lysates (35 ug/lane). GJA3 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-GJA3 antibody

Family: Ion Channel; Subfamily: Connexin

NCBI and Uniprot Product Information

• NCBI GI #: 22779877
• NCBI GeneID: 2700
• NCBI Accession #: NP_068773.2
• NCBI GenBank Nucleotide #: NM_021954.3
• UniProt Accession #: Q9Y6H8; Q0VAB7; Q9H537
• Molecular Weight: 47,410 Da
• NCBI Official Full Name: gap junction alpha-3 protein
• NCBI Official Synonym Full Names: gap junction protein, alpha 3, 46kDa
• NCBI Official Symbol: GJA3
• NCBI Official Synonym Symbols: CX46; CZP3; CTRCT14
• NCBI Protein Information: gap junction alpha-3 protein; connexin 46; connexin-46
• UniProt Protein Name: Gap junction alpha-3 protein
• Protein Family: Gap junction alpha-3 protein
• UniProt Gene Name: GJA3
• UniProt Synonym Gene Names: Cx46
• UniProt Entry Name: CXA3_HUMAN

NCBI Description

The protein encoded by this gene is a connexin and is a component of lens fiber gap junctions. Defects in this gene are a cause of zonular pulverulent cataract type 3 (CZP3). [provided by RefSeq, Jan 2010]

Uniprot Description

GJA3: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJA3 are the cause of cataract zonular pulverulent cataract type 3 (CZP3). A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes. Defects in GJA3 are a cause of cataract autosomal dominant (ADC). Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Belongs to the connexin family. Alpha-type (group II) subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q12.11

Cellular Component: connexon complex; integral to membrane

Molecular Function: gap junction channel activity

Biological Process: cell-cell signaling; visual perception; transport; transmembrane transport

Disease: Cataract 14, Multiple Types