Rabbit anti-Mouse, rat NMDA NR2C Subunit (Ser1244) Polyclonal Antibody | anti-GRIN2C antibody

Anti-Phospho Ser1244 NMDA Receptor, NR2C Subunit

Cat. Num. AAA502150

• Gene Names: Grin2c; NR2C; GluN2C
• Reactivity: Mouse, rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: NMDA NR2C Subunit (Ser1244); Polyclonal Antibody; Anti-Phospho Ser1244 NMDA Receptor; NR2C Subunit; anti-GRIN2C antibody

• Host: Rabbit
• Reactivity: Mouse, rat
• Clonality: Polyclonal
• Specificity: Specific for the ~140k NR2C subunit of the NMDA receptor phosphorylated at Ser1244. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to generate the antibody but not by the corresponding dephospho-peptide.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 1250

• Applicable Applications for anti-GRIN2C antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Synthetic phospho-peptide surrounding the phospho Ser1244 of the NR2C-subunit of rat NMDA receptor.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1244 conjugated to KLH
• Immunogen Species: Rat
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with mouse and rat tissue. It is also expected that the antibody will react with bovine, canine, human and non-human primates as these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996). Phosphorylation of Ser1244 has been shown to regulate NMDA receptor channel function (Chen et al., 2006).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-GRIN2C antibody

Affinity purified rabbit polyclonal antibody

References

• Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.
• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Ebralidze AK, Rossi DJ, Tonegawa S, Slater NT (1996) Modification of NMDA receptor channels and synaptic transmission by targeted disruption of the NR2C gene. J Neurosci 16:5014-5025.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
• Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724.

NCBI and Uniprot Product Information

• NCBI GI #: 148747563
• NCBI GeneID: 24411
• NCBI Accession #: NP_036707.3
• NCBI GenBank Nucleotide #: NM_012575.3
• UniProt Accession #: Q00961
• Molecular Weight: 140
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2C
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 2C
• NCBI Official Symbol: Grin2c
• NCBI Official Synonym Symbols: NR2C; GluN2C
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2C; NMDAR2C; NMDA glutamate receptor; glutamate receptor, ionotropic, NMDA2C; N-methyl D-aspartate receptor subtype 2C; glutamate [NMDA] receptor subunit epsilon-3
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2C
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: Grin2c
• UniProt Synonym Gene Names: GluN2C; NMDAR2C; NR2C
• UniProt Entry Name: NMDE3_RAT

NCBI Description

subunit that in combination with NMDAR1 displays NMDA receptor activity; may contribute to NMDA receptor functional diversity [RGD, Feb 2006]

Uniprot Description

Function: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Interacts with PDZ domains of INADL and DLG4. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes. Ref.3 Ref.4

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily. [View classification]

Sequence caution: The sequence BAA02499.1 differs from that shown. Reason: Erroneous initiation.