Rabbit anti-Mouse, rat COXIV (Ser58) Polyclonal Antibody | anti-COX4i1 antibody

Anti-Phospho-Ser58 COXIV

Cat. Num. AAA502194

• Gene Names: Cox4i1; Cox4; COXIV; Cox4a; AL024441
• Reactivity: Mouse, rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: COXIV (Ser58); Polyclonal Antibody; Anti-Phospho-Ser58 COXIV; anti-COX4i1 antibody

• Host: Rabbit
• Reactivity: Mouse, rat
• Clonality: Polyclonal
• Specificity: Specific for the ~17k COXIV-1 protein phosphorylated at Ser58. Immunolabeling is blocked by the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 169

• Applicable Applications for anti-COX4i1 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of mouse COXIV protein.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH
• Immunogen Species: Mouse
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with mouse and rat tissue.
• Biological Significance: COX, also known as cytochrome c oxidase, has 12 subunits that make up the transmembrane mitochondrial protein. Subunit IV has two isoforms; COXIV-1 and COXIV-2 (Huttermann et al., 2001). COXIV-1 is expressed ubiquitously while COXIV-2 is highly expressed in adult lung and low levels in brain and heart (Huttermann et al., 2001). Phosphorylation of amino acid residue ser58 of the COXIV-1 protein is a PKA-dependent regulation of COX and plays an important role in metabolism and CREB cycle activation (Acin-Perez et al., 2011).
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-COX4i1 antibody

Affinity purified rabbit polyclonal antibody

References

• Acin-Perez R, Gatti D, Bai Y, Manfredi D (2011) Protein Phosphorylation and Prevention of Cytochrome Oxidase Inhibition by ATP: Coupled Mechanisms of Energy Metabolism Regulation. Cell Metabolism 13, 712-719.
• Huttermann M, Kadenbach B, and Grossman LI (2001) Mammalian subunit IV isoforms of cytochrome c oxidase. Gene 267, 111-123.

NCBI and Uniprot Product Information

• NCBI GI #: 6753498
• NCBI GeneID: 12857
• NCBI Accession #: NP_034071.1
• NCBI GenBank Nucleotide #: NM_009941.2
• UniProt Accession #: P19783; Q545A9
• Molecular Weight: 17
• NCBI Official Full Name: cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit IV isoform 1
• NCBI Official Symbol: Cox4i1
• NCBI Official Synonym Symbols: Cox4; COXIV; Cox4a; AL024441
• NCBI Protein Information: cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX IV-1; cytochrome c oxidase, subunit IVa; cytochrome c oxidase polypeptide IV
• UniProt Protein Name: Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• Protein Family: Cytochrome c oxidase
• UniProt Gene Name: Cox4i1
• UniProt Synonym Gene Names: Cox4; Cox4a; COX IV-1
• UniProt Entry Name: COX41_MOUSE

Uniprot Description

Function: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Subcellular location: Mitochondrion inner membrane.

Sequence similarities: Belongs to the cytochrome c oxidase IV family.