Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-CD28 antibody 1C6 Dog peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the mouse IgG1 version of 1C6 (MBS488426, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
CD28 recombinant antibody
Anti-CD28 [1C6]
Reactivity
Dog
Applications
Flow Cytometry, Functional Assay, Functional Assay, ELISA
Purity
Protein A Affinity Purified
Synonyms
CD28; Monoclonal Recombinant Antibody; Anti-CD28 [1C6]; TP44; Costimulatory molecule B7 receptor CD28; T-cell costimulatory molecule CD28; CD28 recombinant antibody
Reactivity
Dog
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
1C6
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Sequence Length
221
Applicable Applications for CD28 recombinant antibody
Flow Cytometry (FC/FACS), FA, In Vivo, Blocking (BL), ELISA (EIA)
Antibody Type
Recombinant Antibody
Antibody Species
Mouse
Original Species Name
Mouse
Immunogen
Canine CD28.
Clone Note
The antibody binds specifically to canine CD28, a self self surface receptor expressed by thymocytes and a subset of natural killer cells. CD28 acts as a receptor for CD80 and CD86, providing a costimulatory signal for the activation of T-cells and the production of interleukins, such as IL-6. The 1C6 monoclonal antibody has antagonistic activity, blocking mixed lymphocyte reactions and cytotoxic T lymphocyte mediated killing. The use of the monoclonal antibody 1C6 for the neutralisation of CD26 signal following T-cell receptor activation is of clinical importance for transplantation therapy, as it has been shown to prevent graft rejection and graft-versus-host disease in the canine model of allogeneic hematopoietic cell transplantation (HCT).
Buffer Composition
PBS only with 0.02% Proclin 300.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-CD28 antibody 1C6 Dog peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the mouse IgG1 version of 1C6 (MBS488426, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the anti-CD28 antibody 1C6 Dog peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the mouse IgG1 version of 1C6 (MBS488426, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Related Product Information for CD28 recombinant antibody
Recombinant monoclonal antibody to CD28. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma 1C6.
Product Categories/Family for CD28 recombinant antibody
References
Scott S. Graves, Diane M. Stone, Carol Loretz, Laura J. Peterson, Marina Lesnikova, Billanna Hwang, George E. Georges, Richard Nash and Rainer Storb. Antagonistic and agonistic anti-canine CD28 monoclonal antibodies: tools for allogeneic transplantation. Transplantation. 2011 Apr 27; 91(8): 833–840. PMID:21343872
Original Published Journal: Transplantation. 2011 Apr 27; 91(8): 833–840.
Original Published Journal: Transplantation. 2011 Apr 27; 91(8): 833–840.
Publication Note: Describes the role of CD28 in the activation of T cells via costimulatory signals, and the blockade of this signal using anti-CD28 monoclonal antibodies such as 1C6. Also demonstrates the advantages of using this method in comparison to cytotoxic T lymphocyte antigen 4 (CTLA-4)-Ig fusion protein by in vivo studies in the dog HCT model.
Original Published Journal: Transplantation. 2011 Apr 27; 91(8): 833–840.
Original Published Journal: Transplantation. 2011 Apr 27; 91(8): 833–840.
Publication Note: Describes the role of CD28 in the activation of T cells via costimulatory signals, and the blockade of this signal using anti-CD28 monoclonal antibodies such as 1C6. Also demonstrates the advantages of using this method in comparison to cytotoxic T lymphocyte antigen 4 (CTLA-4)-Ig fusion protein by in vivo studies in the dog HCT model.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
T-cell costimulatory molecule CD28
Protein Family
Similar Products
Product Notes
The CD28 (Catalog #AAA488426) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD28 [1C6] reacts with Dog and may cross-react with other species as described in the data sheet. AAA Biotech's CD28 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FA, In Vivo, Blocking (BL), ELISA (EIA). Researchers should empirically determine the suitability of the CD28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD28, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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